PMID- 7822787
OWN - NLM
STAT- MEDLINE
DCOM- 19950216
LR  - 20081121
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 154
IP  - 3
DP  - 1995 Feb 1
TI  - Memory B lymphocytes in human tonsil do not express surface IgD.
PG  - 1105-13
AB  - To clarify the phenotype of memory B lymphocytes, we have determined the 
      frequency of somatic hypermutations in purified tonsil B cell populations. Our 
      particular interest was the controversial question of whether any memory B-cells 
      express IgD. Ig heavy chain gene rearrangements that used the nonpolymorphic VH6 
      gene were amplified by PCR, cloned, and sequenced. All eight sequences obtained 
      from the surface IgD (sIgD)-negative fraction contained point mutations, with 
      frequency of one mutation in every 24 bases of sequence. In contrast, only 4 of 
      12 sequences obtained from the sIgD-positive fraction contained point mutations, 
      with a mutation frequency of one in 600. This frequency was similar to that found 
      for cord blood B cells (one in 550), a population that does not contain memory B 
      cells. These results indicate that although memory B cells are present in the 
      sIgD-negative fraction, no memory cells can be detected in the sIgD-positive 
      fraction of tonsil B lymphocytes.
FAU - Nicholson, I C
AU  - Nicholson IC
AD  - Department of Clinical Immunology, Flinders Medical Centre, Bedford Park, 
      Australia.
FAU - Brisco, M J
AU  - Brisco MJ
FAU - Zola, H
AU  - Zola H
LA  - eng
SI  - GENBANK/L32538
SI  - GENBANK/L32545
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Immunoglobulin D)
RN  - 0 (Immunoglobulin Heavy Chains)
RN  - 0 (Immunoglobulin Variable Region)
RN  - 9007-49-2 (DNA)
SB  - IM
GS  - V<down>H</down>6
MH  - Amino Acid Sequence
MH  - B-Lymphocyte Subsets/immunology
MH  - Base Sequence
MH  - Cloning, Molecular
MH  - DNA/analysis
MH  - Flow Cytometry
MH  - Gene Rearrangement, B-Lymphocyte/*genetics
MH  - Humans
MH  - Immunoglobulin D/*genetics/immunology
MH  - Immunoglobulin Heavy Chains/*genetics
MH  - Immunoglobulin Variable Region/*genetics
MH  - Immunologic Memory/genetics
MH  - Molecular Sequence Data
MH  - Mutation/*genetics
MH  - Palatine Tonsil/cytology
MH  - Polymerase Chain Reaction
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1995 Feb 1;154(3):1105-13.