PMID- 7822801
OWN - NLM
STAT- MEDLINE
DCOM- 19950216
LR  - 20131121
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 154
IP  - 3
DP  - 1995 Feb 1
TI  - Human granulocytes contain an opiate alkaloid-selective receptor mediating 
      inhibition of cytokine-induced activation and chemotaxis.
PG  - 1323-30
AB  - Human peripheral blood granulocytes previously were found to contain opioid delta 
      2-receptors mediating stimulation by opioid peptides of chemotaxis. Studies 
      presented in this work indicate that granulocytes also contain opiate 
      alkaloid-selective, opioid peptide-insensitive receptors mediating inhibition by 
      morphine and other opiates of cytokine-induced activation and chemotaxis. Binding 
      studies with [3H]morphine and [3H]diprenorphine ([3H]DPN) indicated the presence 
      of receptor sites, at considerable density with affinities and selectivity for 
      opiates comparable with those of the mu 3-receptor of human peripheral blood 
      monocytes (macrophages). The influence of the guanosine 5'-triphosphate (GTP) 
      analogue GppNHp on binding indicated that the granulocyte receptor was linked to 
      a G protein. Morphine but not opioid peptides interfered with activation and/or 
      chemotaxis of the granulocytes induced by TNF-alpha, IL-1 alpha, IL-8, and FMLP 
      (chemotactic peptide). These effects of morphine were blocked by the antagonist 
      naloxone. Levorphanol inhibited TNF-alpha-induced activation, and also 
      potentiated the inhibition by morphine. Furthermore, in binding assays, 
      levorphanol enhanced the affinity of the receptor for morphine. Dextrorphan had 
      no effect on activation or chemotaxis, and it also had no effect on binding, 
      indicative of stereoselectivity for the effect of levorphanol. It is concluded 
      that human granulocytes contain opiate alkaloid-selective mu 3-receptors that 
      mediate inhibitory effects of morphine on cellular activation by cytokines.
FAU - Makman, M H
AU  - Makman MH
AD  - Department of Biochemistry, Albert Einstein College of Medicine, Bronx, NY 10461.
FAU - Bilfinger, T V
AU  - Bilfinger TV
FAU - Stefano, G B
AU  - Stefano GB
LA  - eng
GR  - NIDA 09010/DA/NIDA NIH HHS/United States
GR  - NIDA 17138/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Cytokines)
RN  - 0 (Narcotics)
RN  - 0 (Receptors, Opioid)
RN  - 0 (Receptors, Opioid, delta)
RN  - 0 (Receptors, Opioid, mu)
RN  - 27618J1N2X (Levorphanol)
RN  - 34273-04-6 (Guanylyl Imidodiphosphate)
RN  - 76I7G6D29C (Morphine)
SB  - IM
MH  - Adult
MH  - Binding, Competitive
MH  - Chemotaxis, Leukocyte/drug effects/physiology
MH  - Corpus Striatum/metabolism
MH  - Cytokines/*antagonists & inhibitors
MH  - Granulocytes/*chemistry
MH  - Guanylyl Imidodiphosphate/pharmacology
MH  - Humans
MH  - Levorphanol/pharmacology
MH  - Male
MH  - Morphine/*pharmacology
MH  - Narcotics/pharmacology
MH  - Radioligand Assay
MH  - Receptors, Opioid/*analysis/drug effects/*physiology
MH  - Receptors, Opioid, delta/analysis
MH  - Receptors, Opioid, mu/analysis
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
PST - ppublish
SO  - J Immunol. 1995 Feb 1;154(3):1323-30.