PMID- 7823026
OWN - NLM
STAT- MEDLINE
DCOM- 19950216
LR  - 20220317
IS  - 0022-0949 (Print)
IS  - 0022-0949 (Linking)
VI  - 196
DP  - 1994 Nov
TI  - Biochemistry and molecular biology of the vesicular monoamine transporter from 
      chromaffin granules.
PG  - 251-62
AB  - Prior to secretion, monoamines (catecholamines, serotonin, histamine) are 
      concentrated from the cytoplasm into vesicles by vesicular monoamine transporters 
      (VMAT). These transporters also carry non-physiological compounds, e.g. the 
      neurotoxin methyl-4-phenylpyridinium. VMAT acts as an electrogenic antiporter 
      (exchanger) of protons and monoamines, using a proton electrochemical gradient. 
      Vesicular transport is inhibited by specific ligands, including tetrabenazine, 
      ketanserin and reserpine. The mechanism of transport and the biochemistry of VMAT 
      have been analyzed with the help of these tools, using mainly the chromaffin 
      granules from bovine adrenal glands as a source of transporter. Although 
      biochemical studies did not suggest a multiplicity of VMATs, two homologous but 
      distinct VMAT genes have recently been cloned from rat, bovine and human adrenal 
      glands. The VMAT proteins are predicted to possess 12 transmembrane segments, 
      with both extremities lying on the cytoplasmic side. They possess N-glycosylation 
      sites in a putative luminal loop and phosphorylation sites in cytoplasmic 
      domains. In rat, VMAT1 is expressed in the adrenal gland whereas VMAT2 is 
      expressed in the brain. In contrast, we found that the bovine adrenal gland 
      expressed both VMAT1 and VMAT2. VMAT2 corresponds to the major transporter of 
      chromaffin granules, as shown by partial peptidic sequences of the purified 
      protein and by a pharmacological analysis of the transport obtained in 
      transfected COS cells (COS cells are monkey kidney cells possessing the ability 
      to replicate SV-40-origin-containing plasmids). We discuss the possibility that 
      VMAT1 may be specifically addressed to large secretory granules vesicles, whereas 
      VMAT2 may also be addressed to small synaptic vesicles; species differences would 
      then reflect the distinct physiological roles of the small synaptic vesicles in 
      the adrenal gland.
FAU - Henry, J P
AU  - Henry JP
AD  - CNRS URA 1112, Neurobiologie Physico-Chimique, Institut de Biologie 
      Physico-Chimique, Paris, France.
FAU - Botton, D
AU  - Botton D
FAU - Sagne, C
AU  - Sagne C
FAU - Isambert, M F
AU  - Isambert MF
FAU - Desnos, C
AU  - Desnos C
FAU - Blanchard, V
AU  - Blanchard V
FAU - Raisman-Vozari, R
AU  - Raisman-Vozari R
FAU - Krejci, E
AU  - Krejci E
FAU - Massoulie, J
AU  - Massoulie J
FAU - Gasnier, B
AU  - Gasnier B
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Review
PL  - England
TA  - J Exp Biol
JT  - The Journal of experimental biology
JID - 0243705
RN  - 0 (Glycoproteins)
RN  - 0 (Membrane Glycoproteins)
RN  - 0 (Membrane Transport Proteins)
RN  - 0 (Neuropeptides)
RN  - 0 (Neurotransmitter Agents)
RN  - 0 (SLC18A1 protein, human)
RN  - 0 (SLC18A2 protein, human)
RN  - 0 (Slc18a1 protein, rat)
RN  - 0 (Slc18a2 protein, rat)
RN  - 0 (Vesicular Biogenic Amine Transport Proteins)
RN  - 0 (Vesicular Monoamine Transport Proteins)
SB  - IM
GS  - VMAT
GS  - VMAT1
GS  - VMAT2
MH  - Adrenal Medulla/*metabolism
MH  - Animals
MH  - Biological Transport
MH  - Cattle
MH  - Cell Line
MH  - Chlorocebus aethiops
MH  - Chromaffin Granules/*metabolism
MH  - Gene Expression
MH  - Glycoproteins/biosynthesis/*genetics/*metabolism
MH  - Humans
MH  - Kidney
MH  - *Membrane Glycoproteins
MH  - *Membrane Transport Proteins
MH  - Models, Biological
MH  - Neurons/metabolism
MH  - *Neuropeptides
MH  - Neurotransmitter Agents/*metabolism
MH  - Rats
MH  - Transfection
MH  - Vesicular Biogenic Amine Transport Proteins
MH  - Vesicular Monoamine Transport Proteins
RF  - 49
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1242/jeb.196.1.251 [doi]
PST - ppublish
SO  - J Exp Biol. 1994 Nov;196:251-62. doi: 10.1242/jeb.196.1.251.