PMID- 7823131
OWN - NLM
STAT- MEDLINE
DCOM- 19950214
LR  - 20191101
IS  - 0270-6474 (Print)
IS  - 1529-2401 (Electronic)
IS  - 0270-6474 (Linking)
VI  - 15
IP  - 1 Pt 1
DP  - 1995 Jan
TI  - Activation of a metabotropic glutamate receptor increases intracellular calcium 
      concentrations in neurons of the avian cochlear nucleus.
PG  - 214-22
AB  - Metabotropic glutamate receptors have been shown to stimulate 
      phosphatidylinositol metabolism, and subsequently liberate Ca2+ from 
      intracellular stores, in a variety of tissue and cell types. We previously 
      demonstrated that glutamate could stimulate phosphatidylinositol metabolism, 
      generating inositol-1,4,5-trisphosphate (IP3), in isolated cochlear nucleus 
      tissue from the chick. Using the calcium indicator dye fura-2 and ratiometric 
      fluorescent imaging, this study examined the ability of glutamate and its analogs 
      to liberate Ca2+ from intracellular stores of neurons of the avian cochlear 
      nucleus, and qualitatively characterized the pharmacological profile of such an 
      action. In normal, Ca(2+)-containing medium, glutamate, kainate (KA), 
      alpha-amino-3-hydroxy-5-methyl-isoxazole-4-propionate (AMPA), NMDA, quisqualate 
      (QUIS), and (+/-)-aminocyclopentane-trans-dicarboxylate (ACPD) elicited increases 
      in intracellular calcium concentrations ([Ca2+]i). In the absence of external 
      Ca2+, glutamate, quisqualate, and ACPD evoked increases in [Ca2+]i. In normal 
      medium, the ionotropic glutamate receptor antagonist 
      6-cyano-7-nitroquinoxaline-2,3-dione (CNQX) and the NMDA receptor antagonist 
      2-amino-5-phosphonovalerate (APV) attenuated but did not abolish the 
      glutamate-evoked response and had no effect on the ACPD-evoked response. The 
      putative metabotropic glutamate receptor antagonist 2-amino-3-phosphonopropionate 
      (AP3) was without effect on the glutamate- and ACPD-evoked increases in [Ca2+]i 
      in Ca(2+)-free medium. We conclude that a metabotropic glutamate receptor (mGluR) 
      is present on cochlear nucleus neurons and is able to stimulate the 
      phosphatidylinositol metabolism--Ca2+ signal transduction cascade.
FAU - Zirpel, L
AU  - Zirpel L
AD  - Department of Physiology and Biophysics, School of Medicine, University of 
      Washington, Seattle 98195.
FAU - Lachica, E A
AU  - Lachica EA
FAU - Rubel, E W
AU  - Rubel EW
LA  - eng
GR  - DC00395/DC/NIDCD NIH HHS/United States
GR  - GM07108/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Neurosci
JT  - The Journal of neuroscience : the official journal of the Society for 
      Neuroscience
JID - 8102140
RN  - 0 (Receptors, Metabotropic Glutamate)
RN  - 0TQU7668EI (Cycloleucine)
RN  - 111900-32-4 (1-amino-1,3-dicarboxycyclopentane)
RN  - 3KX376GY7L (Glutamic Acid)
RN  - 8OC22C1B99 (Quisqualic Acid)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Calcium/*metabolism
MH  - Chick Embryo
MH  - Cochlear Nucleus/*metabolism
MH  - Cycloleucine/analogs & derivatives/pharmacology
MH  - Glutamic Acid/pharmacology
MH  - In Vitro Techniques
MH  - Intracellular Membranes/*metabolism
MH  - Neurons/*metabolism
MH  - Osmolar Concentration
MH  - Quisqualic Acid/pharmacology
MH  - Receptors, Metabotropic Glutamate/agonists/*metabolism
PMC - PMC6578309
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
PMCR- 1995/07/01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
PHST- 1995/07/01 00:00 [pmc-release]
AID - 10.1523/JNEUROSCI.15-01-00214.1995 [doi]
PST - ppublish
SO  - J Neurosci. 1995 Jan;15(1 Pt 1):214-22. doi: 10.1523/JNEUROSCI.15-01-00214.1995.