PMID- 7823955
OWN - NLM
STAT- MEDLINE
DCOM- 19950216
LR  - 20210526
IS  - 0270-7306 (Print)
IS  - 1098-5549 (Electronic)
IS  - 0270-7306 (Linking)
VI  - 15
IP  - 2
DP  - 1995 Feb
TI  - Multiple response elements in the Sex-lethal early promoter ensure its 
      female-specific expression pattern.
PG  - 904-17
AB  - The choice of sexual identity in somatic tissues of the fruit fly Drosophila 
      melanogaster is determined early in embryogenesis by the X-chromosome-to-autosome 
      (X/A) ratio. The system that signals the X/A ratio selects the sexual development 
      pathway by determining the activity state of the binary switch Sex-lethal (Sxl). 
      In 2X/2A animals, the X/A signalling system turns the Sxl gene on, ultimately 
      activating an RNA-splicing autoregulatory feedback loop which serves to maintain 
      the female state during the remainder of development. In 1X/2A animals, this 
      autoregulatory feedback loop is not activated and the male state is subsequently 
      maintained by the default splicing machinery. In the studies reported here, we 
      have examined how the X/A signalling system controls the initial choice of sexual 
      identity through its action on a special early embryonic Sxl promoter, Sxl-Pe. We 
      show that in the early embryo, the activity of Sxl-Pe is controlled in a highly 
      dose-sensitive fashion by the genes on the X chromosome that function as 
      numerator elements and by genes located on the autosomes that function as 
      denominator elements. Functional dissection of Sxl-Pe indicates that activating 
      the promoter in females requires the cumulative action of multiple numerator 
      genes which appear to exert their effects through reiterated cis-acting target 
      sites in the promoter. Conversely, maintaining the promoter silent in males 
      requires the repressive activities of denominator genes, and at least one of the 
      denominator genes also appears to function through target sequences within the 
      promoter.
FAU - Estes, P A
AU  - Estes PA
AD  - Department of Molecular Biology, Princeton University, New Jersey 08544.
FAU - Keyes, L N
AU  - Keyes LN
FAU - Schedl, P
AU  - Schedl P
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Mol Cell Biol
JT  - Molecular and cellular biology
JID - 8109087
SB  - IM
GS  - Scl
GS  - Scl-Pe
GS  - sis-a
GS  - sis-b
MH  - Animals
MH  - Crosses, Genetic
MH  - Drosophila melanogaster/genetics/*physiology
MH  - Female
MH  - Gene Deletion
MH  - *Gene Expression Regulation
MH  - *Genes, Insect
MH  - *Genes, Lethal
MH  - Male
MH  - Models, Genetic
MH  - Multigene Family
MH  - *Promoter Regions, Genetic
MH  - Sex Characteristics
MH  - Signal Transduction
MH  - Transformation, Genetic
MH  - *X Chromosome
PMC - PMC231975
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
PMCR- 1995/02/01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
PHST- 1995/02/01 00:00 [pmc-release]
AID - 10.1128/MCB.15.2.904 [doi]
PST - ppublish
SO  - Mol Cell Biol. 1995 Feb;15(2):904-17. doi: 10.1128/MCB.15.2.904.