PMID- 7824270
OWN - NLM
STAT- MEDLINE
DCOM- 19950214
LR  - 20071114
IS  - 0950-9232 (Print)
IS  - 0950-9232 (Linking)
VI  - 10
IP  - 1
DP  - 1995 Jan 5
TI  - Transforming growth factor-beta 1 inhibits cyclin D1 expression in intestinal 
      epithelial cells.
PG  - 177-84
AB  - Transforming growth factor-beta 1 (TGF-beta 1) inhibits most epithelial cell 
      types by blocking cell cycle progression during the G1 phase. D cyclins are 
      normally expressed during G1 and are regulators of G1 progression. One of the 
      crucial functions of D cyclins is their ability to bind to a cyclin-dependent 
      kinase (Cdk4). In mink lung epithelial cells, TGF-beta 1 inhibits Cdk4 
      expression. We have measured cell cycle progression and D cyclins and Cdk4 
      expression in non-transformed rat intestinal epithelial cell lines (IEC-6 and 
      RIE-1) after TGF-beta 1 treatment. In exponentially growing cultures, TGF-beta 1 
      blocked DNA synthesis and suppressed cyclin D1 mRNA and protein expression, 
      whereas the levels of cyclins D2, D3 and Cdk4 remained relatively unchanged. 
      TGF-beta 1 was also added to G0-synchronized IEC-6 cells after serum stimulation. 
      TGF-beta 1 prevention of G1 progression was associated with an inhibition of 
      cyclin D1 protein expression. Cyclin D3 levels were not affected by TGF-beta 1 
      during G1 traverse. Our results suggest that cyclin D/Cdk4 is a crucial target of 
      TGF-beta 1 and that regulation of this kinase is mediated through cyclin D1 in 
      intestinal epithelial cells.
FAU - Ko, T C
AU  - Ko TC
AD  - Department of Surgery, University of Texas Medical Branch, Galveston 77555.
FAU - Sheng, H M
AU  - Sheng HM
FAU - Reisman, D
AU  - Reisman D
FAU - Thompson, E A
AU  - Thompson EA
FAU - Beauchamp, R D
AU  - Beauchamp RD
LA  - eng
GR  - CA 24347/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Oncogene
JT  - Oncogene
JID - 8711562
RN  - 0 (Cyclins)
RN  - 0 (Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 136601-57-5 (Cyclin D1)
SB  - IM
MH  - Animals
MH  - Cell Division
MH  - Cyclin D1
MH  - Cyclins/*antagonists & inhibitors/genetics
MH  - Epithelial Cells
MH  - Epithelium/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Intestine, Small/cytology/*metabolism
MH  - Oncogene Proteins/*antagonists & inhibitors/genetics
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 1995/01/05 00:00
MHDA- 1995/01/05 00:01
CRDT- 1995/01/05 00:00
PHST- 1995/01/05 00:00 [pubmed]
PHST- 1995/01/05 00:01 [medline]
PHST- 1995/01/05 00:00 [entrez]
PST - ppublish
SO  - Oncogene. 1995 Jan 5;10(1):177-84.