PMID- 7825062
OWN - NLM
STAT- MEDLINE
DCOM- 19950216
LR  - 20191023
IS  - 0740-7750 (Print)
IS  - 0740-7750 (Linking)
VI  - 20
IP  - 5
DP  - 1994 Sep
TI  - Analysis of region-specific library constructed by sequence-independent 
      amplification of microdissected fragments surrounding weaver (wv) gene on mouse 
      chromosome 16.
PG  - 401-8
AB  - The C3-C4 region of mouse chromosome 16 was microdissected and amplified directly 
      by sequence-independent amplification (SIA). The SIA product was proved to 
      originate from the microdissected region by fluorescence in situ hybridization 
      (FISH) and was cloned into the PCR II vector (mean insert size 506 bp). Colony 
      hybridization showed that about 59% of the clones contained either unique or low 
      copy number sequences. Southern blot analysis of 100 unique clones demonstrated 
      that 50 clones hybridized with single (33 clones) or multiple (17 clones) bands 
      on blots of DNA from a hamster-mouse hybrid cell line that contains mouse 
      chromosome 16, 13 clones hybridized with mouse but not with the hamster-mouse 
      hybrid DNA, 19 clones contained repetitive sequences, and the remaining 18 clones 
      failed to yield bands. One third of the 100 unique clones hybridized to human 
      genomic DNA. Thirty-three clones were sequenced. None of them was found in 
      GenBank. Our results demonstrate that this relatively simple method of 
      microdissection and cloning can produce a library of good quality.
FAU - Wei, J
AU  - Wei J
AD  - Department of Pathology, Indiana University School of Medicine, Indianapolis 
      46202-5251.
FAU - Dlouhy, S R
AU  - Dlouhy SR
FAU - Zhu, J
AU  - Zhu J
FAU - Ghetti, B
AU  - Ghetti B
FAU - Hodes, M E
AU  - Hodes ME
LA  - eng
GR  - P10 NS27613/NS/NINDS NIH HHS/United States
GR  - R01 NS14426/NS/NINDS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Somat Cell Mol Genet
JT  - Somatic cell and molecular genetics
JID - 8403568
RN  - 0 (DNA Primers)
RN  - 0 (Genetic Markers)
SB  - IM
GS  - wv
MH  - Animals
MH  - Base Sequence
MH  - *Chromosome Mapping
MH  - Cloning, Molecular
MH  - Cricetinae
MH  - DNA Primers
MH  - Genetic Markers
MH  - *Genomic Library
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Molecular Sequence Data
MH  - *Polymerase Chain Reaction
MH  - Repetitive Sequences, Nucleic Acid
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
AID - 10.1007/BF02257457 [doi]
PST - ppublish
SO  - Somat Cell Mol Genet. 1994 Sep;20(5):401-8. doi: 10.1007/BF02257457.