PMID- 7826941
OWN - NLM
STAT- MEDLINE
DCOM- 19950222
LR  - 20190512
IS  - 0953-8178 (Print)
IS  - 0953-8178 (Linking)
VI  - 6
IP  - 10
DP  - 1994 Oct
TI  - Influence of human leukocyte antigen genes on TCR V gene segment frequencies.
PG  - 1497-504
AB  - Human leukocyte antigen (HLA)-dependent selection mechanisms exerted during 
      thymic maturation are supposed to be main contributing factors to the genetic 
      predetermination of the TCR repertoire and may have a detectable effect on adult 
      peripheral blood lymphocyte V segment frequencies. Here, we analyzed whether 
      polymorphic or non-polymorphic HLA determinants are associated with selected 
      expression of some V gene segment specificities. We first examined the reactivity 
      of 17 V segment specific mAb on purified CD4+ and CD8+ cell fractions in 10 
      unrelated people. We found a significant overexpression of only three V segment 
      products (V beta 2, V beta 5.1 and V beta 6.7) in CD4+ and none in CD8+ cell 
      fractions in most individuals. Skewing of certain V beta segments by 
      non-polymorphic HLA determinants (i.e. class II for CD4+ and class I for CD8+ 
      cells) is therefore more limited (3/17) than previously thought. Considering the 
      effects of polymorphic HLA determinants, we compared TCR V segment frequencies in 
      HLA-identical siblings to sibling pairs who differ at one or both HLA haplotypes, 
      using 13 V beta specific mAb. In pairwise comparisons, we found that the HLA 
      complex had no detectable effect on TCR repertoire in five large families with 
      multiple siblings. Together, these observations suggest that HLA-predicted 
      selection mechanisms exerted during thymic maturation might not have a 
      predominant influence shaping the TCR repertoire of normal adults.
FAU - Genevee, C
AU  - Genevee C
AD  - Laboratoire d'Immunologie Cellulaire, INSERM U 333, Institut Gustave Roussy, 
      Villejuif, France.
FAU - Farace, F
AU  - Farace F
FAU - Chung, V
AU  - Chung V
FAU - Diu, A
AU  - Diu A
FAU - Raffoux, C
AU  - Raffoux C
FAU - Charron, D
AU  - Charron D
FAU - Hercend, T
AU  - Hercend T
FAU - Triebel, F
AU  - Triebel F
LA  - eng
PT  - Journal Article
PL  - England
TA  - Int Immunol
JT  - International immunology
JID - 8916182
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (HLA Antigens)
RN  - 0 (Receptors, Antigen, T-Cell, alpha-beta)
SB  - IM
MH  - Antibodies, Monoclonal/immunology
MH  - CD4-Positive T-Lymphocytes/immunology
MH  - CD8-Positive T-Lymphocytes/immunology
MH  - Flow Cytometry
MH  - Gene Frequency
MH  - HLA Antigens/biosynthesis/*genetics/immunology
MH  - Haplotypes/*genetics
MH  - Humans
MH  - Polymerase Chain Reaction
MH  - Receptors, Antigen, T-Cell, alpha-beta/biosynthesis/*genetics/immunology
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - 10.1093/intimm/6.10.1497 [doi]
PST - ppublish
SO  - Int Immunol. 1994 Oct;6(10):1497-504. doi: 10.1093/intimm/6.10.1497.