PMID- 7827118
OWN - NLM
STAT- MEDLINE
DCOM- 19950222
LR  - 20190610
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1254
IP  - 2
DP  - 1995 Jan 20
TI  - Effect of transforming growth factor-beta on lipoprotein lipase in rat 
      mesenchymal heart cell cultures.
PG  - 140-6
AB  - The effect of recombinant transforming growth factor-beta 2 (rTGF-beta 2) on 
      lipoprotein lipase (LPL) synthesis was studied in mesenchymal rat heart cell 
      cultures. Addition of rTGF-beta 2 to culture medium containing 20% serum resulted 
      in a time-dependent decrease in LPL activity. With 10 ng/ml a 30% fall occurred 
      after 12 h and only 20% of enzyme activity remained after 24 h with 5 or 10 
      ng/ml. The minimal effective dose of rTGF-beta 2 was 0.1 ng/ml and a 20% decrease 
      occurred after exposure for 24 h. Antibodies specific to TGF-beta 2 blocked this 
      effect. The decrease in enzymic activity was accompanied by a decrease in enzyme 
      mass and LPL mRNA. Addition of rTGF-beta 2 was effective only during the first 
      week in culture, when enzyme activity was increasing but not after 12 days when 
      the cultures were overconfluent, and the enzyme activity was high.
FAU - Friedman, G
AU  - Friedman G
AD  - Lipid Research Laboratory, Hadassah University Hospital, Jerusalem, Israel.
FAU - Ben-Yehuda, A
AU  - Ben-Yehuda A
FAU - Ben-Naim, M
AU  - Ben-Naim M
FAU - Matsa, D
AU  - Matsa D
FAU - Stein, O
AU  - Stein O
FAU - Stein, Y
AU  - Stein Y
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (Culture Media)
RN  - 0 (RNA, Messenger)
RN  - 0 (Recombinant Proteins)
RN  - 0 (Transforming Growth Factor beta)
RN  - EC 3.1.1.34 (Lipoprotein Lipase)
SB  - IM
MH  - Animals
MH  - Blotting, Northern
MH  - Cells, Cultured
MH  - Culture Media
MH  - Kinetics
MH  - Lipoprotein Lipase/antagonists & inhibitors/genetics/*metabolism
MH  - Mesoderm/*enzymology
MH  - Myocardium/*enzymology
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Recombinant Proteins/pharmacology
MH  - Transforming Growth Factor beta/*pharmacology
EDAT- 1995/01/20 00:00
MHDA- 1995/01/20 00:01
CRDT- 1995/01/20 00:00
PHST- 1995/01/20 00:00 [pubmed]
PHST- 1995/01/20 00:01 [medline]
PHST- 1995/01/20 00:00 [entrez]
AID - 0005-2760(94)00175-X [pii]
AID - 10.1016/0005-2760(94)00175-x [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 1995 Jan 20;1254(2):140-6. doi: 
      10.1016/0005-2760(94)00175-x.