PMID- 7829106
OWN - NLM
STAT- MEDLINE
DCOM- 19950217
LR  - 20071114
IS  - 0888-7543 (Print)
IS  - 0888-7543 (Linking)
VI  - 23
IP  - 1
DP  - 1994 Sep 1
TI  - Cloning, sequencing, and mapping of the human chromosome 14 heat shock protein 
      gene (HSPA2).
PG  - 85-93
AB  - A genomic clone for the human heat shock protein (HSP) 70 gene located on 
      chromosome 14 was isolated and sequenced. The gene, designated HSPA2, has a 
      single open reading frame of 1917 bp that encodes a 639-amino acid protein with a 
      predicted molecular weight of 70,030 Da. Analysis of the sequence indicates that 
      HSPA2 is the human homologue of the murine Hsp70-2 gene with 91.7% identity in 
      the nucleotide coding sequence and 98.2% in the corresponding amino acid 
      sequence. HSPA2 has less amino acid homology to other members of the human HSP70 
      gene family, 83.3% to the heat-inducible HSP70-1 gene and 86.1% with the human 
      heat shock cognate gene HSC70. HSPA2 is constitutively expressed in most tissues, 
      with very high levels in testis and skeletal muscle. Significant but lower levels 
      are also expressed in ovary, small intestine, colon, brain, placenta, and kidney. 
      A yeast artificial chromosome (YAC) clone containing HSPA2 (YAC741H4) that also 
      contained the polymorphic marker D14S63 was identified. This 670-kb YAC was 
      mapped to 14q24.1 by fluorescence in situ hybridization (FISH). Subsequent 
      two-color FISH and genetic mapping placed HSPA2/D14S63 proximal to the markers 
      D14S57 and D14S77.
FAU - Bonnycastle, L L
AU  - Bonnycastle LL
AD  - School of Medicine, University of Washington, Seattle 98195.
FAU - Yu, C E
AU  - Yu CE
FAU - Hunt, C R
AU  - Hunt CR
FAU - Trask, B J
AU  - Trask BJ
FAU - Clancy, K P
AU  - Clancy KP
FAU - Weber, J L
AU  - Weber JL
FAU - Patterson, D
AU  - Patterson D
FAU - Schellenberg, G D
AU  - Schellenberg GD
LA  - eng
SI  - GENBANK/L26336
SI  - GENBANK/M20567
SI  - GENBANK/X15705
SI  - GENBANK/X52330
GR  - AG00029/AG/NIA NIH HHS/United States
GR  - AG05136/AG/NIA NIH HHS/United States
GR  - HG00256/HG/NHGRI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Genomics
JT  - Genomics
JID - 8800135
RN  - 0 (Heat-Shock Proteins)
SB  - IM
GS  - HSP70
GS  - HSPA2
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 14
MH  - Cloning, Molecular
MH  - Gene Expression Regulation
MH  - Genes
MH  - Heat-Shock Proteins/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lod Score
MH  - Molecular Sequence Data
MH  - Open Reading Frames
MH  - Organ Specificity
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
AID - S0888-7543(84)71462-5 [pii]
AID - 10.1006/geno.1994.1462 [doi]
PST - ppublish
SO  - Genomics. 1994 Sep 1;23(1):85-93. doi: 10.1006/geno.1994.1462.