PMID- 7829272
OWN - NLM
STAT- MEDLINE
DCOM- 19950222
LR  - 20190708
IS  - 0020-7136 (Print)
IS  - 0020-7136 (Linking)
VI  - 60
IP  - 4
DP  - 1995 Feb 8
TI  - Vaccination of rabbits with recombinant vaccinia virus carrying the envelope gene 
      of human T-cell lymphotropic virus type I.
PG  - 567-70
AB  - Two groups of 3 rabbits each were immunized with either recombinant vaccinia 
      virus, WR-SFB5env, carrying the human T-cell lymphotropic virus type I (HTLV-I) 
      env gene at the site of the hemagglutinin gene of the WR strain, or control 
      vaccinia virus, HA-WR, lacking the functional hemagglutinin gene. All 6 rabbits 
      responded with anti-vaccinia virus antibodies. WR-SFB5env elicited anti-HTLV-I 
      env antibodies but no vesicular stomatitis virus (HTLV-I) pseudotype neutralizing 
      antibodies in all 3 rabbits. After 10 weeks, the animals were challenged by 
      transfusion of blood from an HTLV-I-infected rabbit. Two of the 3 vaccinated 
      rabbits and all 3 control rabbits became infected with HTLV-I, as indicated by 
      seroconversion and detection of HTLV-I proviral sequences by polymerase chain 
      reaction. The rabbit that had been protected from initial challenge became 
      infected with HTLV-I upon rechallenge 12 weeks after the first challenge. In view 
      of the proven prophylactic effect of passive immunization against HTLV-I, our 
      vaccine trial failed because WR-SFB5env was incapable of inducing neutralizing 
      antibodies against HTLV-I in the immunized animals. It remains to be studied 
      whether cell-mediated immunity such as antibody-dependent cellular cytotoxicity 
      was involved in the temporary protection of I vaccinated rabbit.
FAU - Hakoda, E
AU  - Hakoda E
AD  - Department of Medicine, Kochi Medical School, Japan.
FAU - Machida, H
AU  - Machida H
FAU - Tanaka, Y
AU  - Tanaka Y
FAU - Morishita, N
AU  - Morishita N
FAU - Sawada, T
AU  - Sawada T
FAU - Shida, H
AU  - Shida H
FAU - Hoshino, H
AU  - Hoshino H
FAU - Miyoshi, I
AU  - Miyoshi I
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Int J Cancer
JT  - International journal of cancer
JID - 0042124
RN  - 0 (HTLV-I Antibodies)
RN  - 0 (Vaccines, Synthetic)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Drug Evaluation, Preclinical
MH  - Genes, env/immunology
MH  - HTLV-I Antibodies/*biosynthesis/blood
MH  - HTLV-I Infections/*prevention & control
MH  - Human T-lymphotropic virus 1/*genetics/immunology
MH  - Neutralization Tests
MH  - Polymerase Chain Reaction
MH  - Rabbits
MH  - *Vaccines, Synthetic
MH  - Vaccinia virus/immunology
MH  - Vesicular stomatitis Indiana virus/immunology
MH  - *Viral Vaccines
EDAT- 1995/02/08 00:00
MHDA- 1995/02/08 00:01
CRDT- 1995/02/08 00:00
PHST- 1995/02/08 00:00 [pubmed]
PHST- 1995/02/08 00:01 [medline]
PHST- 1995/02/08 00:00 [entrez]
AID - 10.1002/ijc.2910600423 [doi]
PST - ppublish
SO  - Int J Cancer. 1995 Feb 8;60(4):567-70. doi: 10.1002/ijc.2910600423.