PMID- 7829396
OWN - NLM
STAT- MEDLINE
DCOM- 19950222
LR  - 20190512
IS  - 0910-5050 (Print)
IS  - 1876-4673 (Electronic)
IS  - 0910-5050 (Linking)
VI  - 85
IP  - 11
DP  - 1994 Nov
TI  - Facilitation of autonomous phenotype acquisition in androgen-dependent Shionogi 
      carcinoma 115 cells by transfection of androgen-induced growth factor expression 
      vector.
PG  - 1117-23
AB  - Androgen-induced growth factor (AIGF) is an autocrine growth factor for 
      androgen-dependent SC-3 cells, which is induced by androgen stimuli. To elucidate 
      the mechanism of the progression from hormone-dependent to -independent tumor, we 
      transfected an expression vector of cDNA encoding AIGF into SC-3 cells and 
      established a stable transfectant (A1) expressing AIGF. A1 cells showed enhanced 
      DNA synthesis. This enhanced DNA synthesis was blocked by exposing the cells to 
      AIGF antisense oligonucleotides, heparin, or suramin, indicating that enforced 
      AIGF expression is responsible for the increase in DNA synthesis. However, A1 
      cells did not grow in serum-free medium unless stimulated with androgen. 
      Recloning from A1 cells in semi-solid agar supplemented with fetal calf serum but 
      without androgen quickly generated an autonomous subline that was able to grow 
      rapidly in the serum-free medium irrespective of androgen stimulus. 
      Mock-transfected SC-3 cells failed to form any colony under identical conditions. 
      These results suggest that stable expression of AIGF alone is not sufficient for, 
      but facilitates the conversion of SC-3 cells from androgen-dependent to 
      -independent phenotype.
FAU - Miyashita, Y
AU  - Miyashita Y
AD  - Department of Medicine III, Osaka University Medical School, Suita.
FAU - Koga, M
AU  - Koga M
FAU - Kouhara, H
AU  - Kouhara H
FAU - Tanaka, A
AU  - Tanaka A
FAU - Kishimoto, T
AU  - Kishimoto T
FAU - Sato, B
AU  - Sato B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Japan
TA  - Jpn J Cancer Res
JT  - Japanese journal of cancer research : Gann
JID - 8509412
RN  - 0 (Androgens)
RN  - 0 (Growth Substances)
RN  - 103107-01-3 (Fibroblast Growth Factor 2)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Androgens/*pharmacology
MH  - Animals
MH  - Base Sequence
MH  - Cell Division/drug effects
MH  - DNA/biosynthesis
MH  - Fibroblast Growth Factor 2/genetics
MH  - Growth Substances/genetics/*physiology
MH  - Mammary Neoplasms, Experimental/*pathology
MH  - Mice
MH  - Molecular Sequence Data
MH  - Neoplasms, Hormone-Dependent/*pathology
MH  - Phenotype
MH  - *Transfection
PMC - PMC5919376
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
PMCR- 1994/11/01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
PHST- 1994/11/01 00:00 [pmc-release]
AID - CAE1117 [pii]
AID - 10.1111/j.1349-7006.1994.tb02916.x [doi]
PST - ppublish
SO  - Jpn J Cancer Res. 1994 Nov;85(11):1117-23. doi: 
      10.1111/j.1349-7006.1994.tb02916.x.