PMID- 7829968
OWN - NLM
STAT- MEDLINE
DCOM- 19950223
LR  - 20190516
IS  - 0741-5400 (Print)
IS  - 0741-5400 (Linking)
VI  - 57
IP  - 1
DP  - 1995 Jan
TI  - Ligation of CD23 activates soluble guanylate cyclase in human monocytes via an 
      L-arginine-dependent mechanism.
PG  - 160-7
AB  - Transduction through Fc epsilon R2/CD23 was analyzed in normal human monocytes 
      using immunoglobulin E (IgE)-anti-IgE immune complexes (IgE ICs) and monoclonal 
      antibodies (mAbs) to CD23. Anti-CD23 mAb and IgE IC triggered a time-dependent 
      increase in cGMP and cAMP in interleukin-4-preincubated (CD23+) but not in 
      unstimulated (CD23-) monocytes. Maximal cGMP and cAMP accumulations were observed 
      10 and 20 min, respectively, after the onset of CD23 ligation. The increase in 
      cGMP was inhibited with N omega-monomethyl-L-arginine (L-NMMA), which also 
      partially affected cAMP accumulation. Addition of an anti-CD23 mAb Fab fragment 
      inhibited the IgE IC- and the anti-CD23 mAb-induced cGMP and cAMP accumulation, 
      confirming the engagement of CD23. In addition, IgE IC and anti-CD23 mAb induced, 
      at least in some donors, a production of nitrite that was inhibited in the 
      presence of L-NMMA. Taken together, these findings suggest a possible involvement 
      of the nitric oxide synthase pathway in IgE IC-mediated activation of CD23+ 
      monocytes.
FAU - Paul-Eugene, N
AU  - Paul-Eugene N
AD  - INSERM U 365, Institut Curie, Paris, France.
FAU - Kolb, J P
AU  - Kolb JP
FAU - Sarfati, M
AU  - Sarfati M
FAU - Arock, M
AU  - Arock M
FAU - Ouaaz, F
AU  - Ouaaz F
FAU - Debre, P
AU  - Debre P
FAU - Mossalayi, D M
AU  - Mossalayi DM
FAU - Dugas, B
AU  - Dugas B
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Leukoc Biol
JT  - Journal of leukocyte biology
JID - 8405628
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Antigen-Antibody Complex)
RN  - 0 (Receptors, IgE)
RN  - 207137-56-2 (Interleukin-4)
RN  - 27JT06E6GR (omega-N-Methylarginine)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - 94ZLA3W45F (Arginine)
RN  - E0399OZS9N (Cyclic AMP)
RN  - EC 4.6.1.2 (Guanylate Cyclase)
RN  - H2D2X058MU (Cyclic GMP)
SB  - IM
MH  - Antibodies, Monoclonal/immunology/pharmacology
MH  - Antigen-Antibody Complex/pharmacology
MH  - Arginine/analogs & derivatives/pharmacology/*physiology
MH  - Cells, Cultured
MH  - Cyclic AMP/metabolism
MH  - Cyclic GMP/metabolism
MH  - Enzyme Activation
MH  - Guanylate Cyclase/*metabolism/physiology
MH  - Humans
MH  - Immunoglobulin E/pharmacology
MH  - Interleukin-4/pharmacology
MH  - Monocytes/cytology/*enzymology/physiology
MH  - Nitric Oxide/antagonists & inhibitors/metabolism
MH  - Receptors, IgE/immunology/*physiology
MH  - omega-N-Methylarginine
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1002/jlb.57.1.160 [doi]
PST - ppublish
SO  - J Leukoc Biol. 1995 Jan;57(1):160-7. doi: 10.1002/jlb.57.1.160.