PMID- 7832810
OWN - NLM
STAT- MEDLINE
DCOM- 19950222
LR  - 20061115
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 206
IP  - 3
DP  - 1995 Jan 26
TI  - Full active baculovirus-expressed human monocyte chemoattractant protein 1 with 
      the intact N-terminus.
PG  - 955-61
AB  - The Sf-9 insect cells infected with a recombinant baculovirus integrated with a 
      cDNA encoding human monocyte chemoattractant protein-1 (MCP-1) produced three 
      different MCP-1s, which were isolated by reverse-phase HPLC as 13kDa, 14kDa, and 
      15kDa bands on SDS-PAGE. The heterogeneity between these MCP-1s was ascribed to 
      the different degree of O-glycosylation with Gal beta 1-3GalNAc beta 1-Ser/Thr, 
      while the 13kDa was regarded as carbohydrate-free. The integrity of the N-termius 
      was confirmed by the amino acid sequence analysis. Each MCP-1 isolated showed 
      equipotent monocyte chemotactic activity, which was rather higher than that of 
      the E. coli-derived one.
FAU - Ishii, K
AU  - Ishii K
AD  - Pharmaceutical Discovery Research Laboratories, Teijin Institute for Biomedical 
      Research, Tokyo, Japan.
FAU - Yamagami, S
AU  - Yamagami S
FAU - Tanaka, H
AU  - Tanaka H
FAU - Motoki, M
AU  - Motoki M
FAU - Suwa, Y
AU  - Suwa Y
FAU - Endo, N
AU  - Endo N
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Chemokine CCL2)
RN  - 0 (Chemotactic Factors)
RN  - 0 (Peptide Fragments)
RN  - 0 (Recombinant Proteins)
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Baculoviridae/*genetics
MH  - Base Sequence
MH  - Carbohydrate Sequence
MH  - Chemokine CCL2
MH  - Chemotactic Factors/chemistry/*genetics/metabolism
MH  - Chromatography, High Pressure Liquid
MH  - Electrophoresis, Polyacrylamide Gel
MH  - *Gene Expression
MH  - Glycosylation
MH  - Humans
MH  - Molecular Sequence Data
MH  - Molecular Weight
MH  - Peptide Fragments/*chemistry
MH  - Recombinant Proteins/chemistry/metabolism
MH  - Spodoptera/metabolism
EDAT- 1995/01/26 00:00
MHDA- 1995/01/26 00:01
CRDT- 1995/01/26 00:00
PHST- 1995/01/26 00:00 [pubmed]
PHST- 1995/01/26 00:01 [medline]
PHST- 1995/01/26 00:00 [entrez]
AID - S0006-291X(85)71135-7 [pii]
AID - 10.1006/bbrc.1995.1135 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1995 Jan 26;206(3):955-61. doi: 
      10.1006/bbrc.1995.1135.