PMID- 7834220
OWN - NLM
STAT- MEDLINE
DCOM- 19950228
LR  - 20191101
IS  - 0967-3849 (Print)
IS  - 0967-3849 (Linking)
VI  - 2
IP  - 6
DP  - 1994 Nov
TI  - Condensation behaviour of the human X chromosome in male germ cells and Sertoli 
      cells examined by fluorescence in situ hybridization.
PG  - 439-44
AB  - The chromatin condensation behaviour of the human X chromosome has been studied 
      by fluorescence in situ hybridization (FISH) analysis in germ cells and Sertoli 
      cells of the adult testis, and comparisons are made with previous findings for 
      the human Y chromosome and for chromosome 7. In meiotic prophase, the X 
      chromosome can be seen to extend greatly at zygotene and to contract through 
      pachytene into the sex vesicle. Such extension, which has also been noted for the 
      human Y chromosome at this stage of meiosis, could be a prerequisite for XY 
      pairing and crossing-over. By in situ hybridization analysis, the sex chromosomes 
      of patients with 'Sertoli-cell-only' syndrome appear extremely contracted 
      compared with the normally extended state of those in adult Sertoli cells of 
      fertile men. By contrast, the state of expansion for chromosome 7 in Sertoli 
      cells appears identical for sterile and fertile testes. This could suggest an 
      association between gene-controlled germ cell losses and failure of expansion of 
      the sex chromosome axes. The variable patterns of extension and contraction for 
      the X and Y chromosome axes in germ cells and Sertoli cells might provide 
      underlying clues to patterns of expression noted for sex-linked genes in the 
      human testis.
FAU - Kofman-Alfaro, S
AU  - Kofman-Alfaro S
AD  - MRC Human Genetics Unit, Western General Hospital Edinburgh, UK.
FAU - Speed, R M
AU  - Speed RM
FAU - Boyle, S
AU  - Boyle S
FAU - Chandley, A C
AU  - Chandley AC
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Chromosome Res
JT  - Chromosome research : an international journal on the molecular, supramolecular 
      and evolutionary aspects of chromosome biology
JID - 9313452
RN  - 0 (Oligonucleotide Probes)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Chromosomes, Human, Pair 7
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Infertility, Male/genetics/pathology
MH  - Male
MH  - Molecular Sequence Data
MH  - Oligonucleotide Probes
MH  - Polymerase Chain Reaction
MH  - Reference Values
MH  - Sertoli Cells/*cytology
MH  - Spermatozoa/*cytology
MH  - X Chromosome/*ultrastructure
MH  - Y Chromosome
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1007/BF01552866 [doi]
PST - ppublish
SO  - Chromosome Res. 1994 Nov;2(6):439-44. doi: 10.1007/BF01552866.