PMID- 7834346
OWN - NLM
STAT- MEDLINE
DCOM- 19950302
LR  - 20190614
IS  - 0006-8993 (Print)
IS  - 0006-8993 (Linking)
VI  - 658
IP  - 1-2
DP  - 1994 Sep 26
TI  - Cholecystokinin blockade of emotional stress- and CRF-induced colonic motor 
      alterations in rats: role of the amygdala.
PG  - 232-8
AB  - Intracerebroventricular (i.c.v.) injection of corticotropin-releasing factor 
      (CRF) and emotional stress (ES) induce stimulation of colonic motility in rats, 
      an effect blocked by i.c.v. injection of CCK-8s. This study examined in rats the 
      contribution of the central nucleus of the amygdala (CA) in the blocking effect 
      of CCK-8s on ES and CRF-induced colonic hypermotility. CRF (500 ng/kg, i.c.v.) 
      induced a 73.5% increase in colonic spike burst frequency. Bilateral infusions of 
      1, 5, 10 and 20 ng/kg of CCK-8s in the CA region 10 min prior to CRF i.c.v. 
      injection reduced, in a dose related manner, the CRF-induced stimulation of 
      colonic motility. A 109% increase in colonic spike burst frequency was observed 
      in rats placed in a test cage in which they had previously received electric 
      footshocks (ES). CCK-8s and A-71623, a selective CCK-A receptor agonist, (10, 25 
      and 50 ng/kg) infused bilaterally into the CA, 30 min before ES, significantly 
      reduced this stimulatory effect, while CCK-4 and A-63387, a selective CCK-B 
      receptor agonist (10, 25 and 50 ng/kg), had no effect on such a response. CA 
      lesions by ibotenic acid did not affect ES-induced increase in colonic spike 
      activity. However, CCK-8s (50 ng/kg) microinfused into CA lesioned rats was 
      unable to block the ES-induced stimulation of colonic motility, while CCK-8s 
      i.c.v. injected (100 ng/kg) is still active on the colonic response to ES. These 
      results suggest that CA is a site of interaction of CCK-8s with CRF to block the 
      colonic response to stress and that these effects involve the CCK-A receptor 
      subtype.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Gue, M
AU  - Gue M
AD  - Department of Pharmacology, INRA, Toulouse, France.
FAU - Tekamp, A
AU  - Tekamp A
FAU - Tabis, N
AU  - Tabis N
FAU - Junien, J L
AU  - Junien JL
FAU - Bueno, L
AU  - Bueno L
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Oligopeptides)
RN  - 0OL293AV80 (Tetragastrin)
RN  - 130408-77-4 (A 71623)
RN  - 140933-16-0 (A 63387)
RN  - 2552-55-8 (Ibotenic Acid)
RN  - 9015-71-8 (Corticotropin-Releasing Hormone)
RN  - M03GIQ7Z6P (Sincalide)
SB  - IM
MH  - Amygdala/*physiology
MH  - Animals
MH  - Colon/drug effects
MH  - Corticotropin-Releasing Hormone/*antagonists & inhibitors
MH  - Gastrointestinal Motility/*drug effects
MH  - Ibotenic Acid
MH  - Injections, Intraventricular
MH  - Male
MH  - Oligopeptides/pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Sincalide/*pharmacology
MH  - Stress, Psychological/*physiopathology
MH  - Tetragastrin/analogs & derivatives/pharmacology
EDAT- 1994/09/26 00:00
MHDA- 1994/09/26 00:01
CRDT- 1994/09/26 00:00
PHST- 1994/09/26 00:00 [pubmed]
PHST- 1994/09/26 00:01 [medline]
PHST- 1994/09/26 00:00 [entrez]
AID - S0006-8993(09)90030-0 [pii]
AID - 10.1016/s0006-8993(09)90030-0 [doi]
PST - ppublish
SO  - Brain Res. 1994 Sep 26;658(1-2):232-8. doi: 10.1016/s0006-8993(09)90030-0.