PMID- 7835081
OWN - NLM
STAT- MEDLINE
DCOM- 19950227
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 69
IP  - 1-2
DP  - 1995
TI  - Mapping of an ordered set of 14 cosmids to human chromosome 12p by two-color in 
      situ hybridization.
PG  - 27-32
AB  - To map human chromosome 12p aberrations by fluorescence in situ hybridization 
      (FISH), cosmids were isolated or obtained for 14 known 12p loci (D12S119, 
      D12S158, D12S178, D12S370, D12S380E, A2M, CACNL1A1, FGF6, GAPD, KRAS2, PRB1, PZP, 
      TPI1, and VWF). Using two-color FISH with three labeled probes to interphase 
      nuclei, and to prometaphase chromosomes where possible, the order of these loci 
      was sequentially determined to be pter-D12S158-D12S380E-CACNL1A1-FGF6- 
      D12S370-VWF-GAPD-TPI1-A2M-PZP-PRB1-D12S 178-D12S119-KRAS2-cen. Two cell lines 
      were analyzed with this set of cosmids. The EBV-transformed cell line TA carries 
      a der(12) with a deletion of bands 12p13.1-->p11.2.D12S178, D12S119, and KRAS2 
      were absent in the der(12), whereas the other loci were present. The second cell 
      line, GM01203A, exhibits a balanced t(4;12)(4q25; 12p13.3) with a breakpoint 
      between FGF6 and D12S370.
FAU - Chaffanet, M
AU  - Chaffanet M
AD  - Center for Human Genetics, University of Leuven, Belgium.
FAU - Baens, M
AU  - Baens M
FAU - Aerssens, J
AU  - Aerssens J
FAU - Schoenmakers, E
AU  - Schoenmakers E
FAU - Cassiman, J J
AU  - Cassiman JJ
FAU - Marynen, P
AU  - Marynen P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
SB  - IM
MH  - Animals
MH  - Cell Nucleus/ultrastructure
MH  - Cells, Cultured
MH  - *Chromosome Aberrations
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 12
MH  - *Cosmids
MH  - Hominidae/*genetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Interphase
MH  - Lymphocytes/cytology
MH  - Male
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1159/000133930 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1995;69(1-2):27-32. doi: 10.1159/000133930.