PMID- 7835094
OWN - NLM
STAT- MEDLINE
DCOM- 19950227
LR  - 20180214
IS  - 0301-0171 (Print)
IS  - 0301-0171 (Linking)
VI  - 69
IP  - 1-2
DP  - 1995
TI  - Eigenanalysis of DAPI-stained chromosomes: tools and strategies toward 
      computer-assisted analysis of FISH experiments.
PG  - 81-6
AB  - The fluorescent dye 4',6-diamidino-2-phenylindole (DAPI) is widely used as a 
      chromosome counterstain in fluorescence in situ hybridization (FISH) studies. It 
      produces a Q-banding pattern that allows for both chromosome identification and 
      the assignment of molecular probes to specific chromosome bands. Using a 
      statistical procedure based on eigenanalysis, we have extracted features from 
      digital images of DAPI-stained chromosomes and constructed prototypes of each of 
      the 24 human chromosomes. The features of these prototypes are directly 
      proportional, in intensity profile and band location, to those of real 
      chromosomes. The prototype's intensity profile can be translated into cytogenetic 
      bands to provide a computer-based strategy for chromosome mapping and analysis 
      amenable to automation. Data presented here were obtained using images from the 
      24 human chromosomes and mouse X chromosome. Moreover, the same procedure is 
      general and can be used for the analysis of chromosomes from other species, as 
      well as with banding techniques other than those using DAPI.
FAU - Knapp, R D
AU  - Knapp RD
AD  - Department of Medicine, Baylor College of Medicine, Houston, TX 77030.
FAU - Smith, L C
AU  - Smith LC
FAU - Baldini, A
AU  - Baldini A
LA  - eng
GR  - 5P30HG0210/HG/NHGRI NIH HHS/United States
GR  - 5P50CA58204/CA/NCI NIH HHS/United States
GR  - HL27341/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Switzerland
TA  - Cytogenet Cell Genet
JT  - Cytogenetics and cell genetics
JID - 0367735
RN  - 0 (DNA Probes)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Animals
MH  - Automation
MH  - Chromosome Banding
MH  - Chromosome Mapping
MH  - *Chromosomes, Human
MH  - Computers
MH  - Cosmids
MH  - DNA Probes
MH  - Fluorescent Dyes
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Indoles
MH  - Karyotyping
MH  - Mice
MH  - *X Chromosome
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.1159/000133943 [doi]
PST - ppublish
SO  - Cytogenet Cell Genet. 1995;69(1-2):81-6. doi: 10.1159/000133943.