PMID- 7835547
OWN - NLM
STAT- MEDLINE
DCOM- 19950301
LR  - 20190909
IS  - 0272-0590 (Print)
IS  - 0272-0590 (Linking)
VI  - 23
IP  - 3
DP  - 1994 Oct
TI  - Nongenotoxic effects of polycyclic aromatic hydrocarbons and their oxygenation 
      by-products on the intercellular communication of rat liver epithelial cells.
PG  - 470-5
AB  - Since polycyclic aromatic hydrocarbons (PAHs) are known to have epigenetic 
      effects, we evaluated the effect of the parent chemical and the ozonated products 
      on in vitro cell to cell communication bioassays which measures a nongenotoxic 
      event. The scrape loading/dye transfer (SL/DT) technique was used to determine 
      the effect of the following PAHs on gap-junction intercellular communication 
      (GJIC): fluorene, 1-methyl-fluorene, fluoranthene, anthracene, 
      9-methyl-anthracene, phenanthrene, pyrene, benzo(a)pyrene, and benzo(e)pyrene. 
      The methylated PAHs were more inhibitory to GJIC than the unmethylated 
      counterparts. Fluoranthene, which has an additional ring added to fluorene, was 
      more effective in inhibiting GJIC than fluorene. The three-ringed PAHs were also 
      more inhibitory than the four- and five-ringed PAHs. A time-course study of 
      fluoranthene and of pyrene resulted in maximal inhibition occurring within 30 min 
      of incubation with the cells. The cells recovered from the inhibition within 1 hr 
      after fluoranthene and pyrene were removed from the cell culture medium. Pyene, 
      vbenzo(a)pyrene, fluorene, and fluoranthene were ozonated until the parent 
      compound was completely eliminated as determined by reverse-phase high-pressure 
      liquid chromatography (RP-HPLC). An increased level of inhibition of GJIC was 
      observed for the ozonated mixtures of by-products of pyrene, fluoranthene, and 
      benzo(a)pyrene, but not for fluorene, as monitored with the SL/DT technique. The 
      products of the ozonated pyrene mixture were fractionated and collected by 
      RP-HPLC. Each fraction was found to be inhibitory to GJIC as monitored by 
      fluorescence recovery after photobleaching. In conclusion, current treatment 
      technologies, such as ozonation or biologically based oxidations and 
      methylations, do not necessarily eliminate toxicity.(ABSTRACT TRUNCATED AT 250 
      WORDS)
FAU - Upham, B L
AU  - Upham BL
AD  - Department of Civil & Environmental Engineering, Michigan State University, East 
      Lansing 48824.
FAU - Masten, S J
AU  - Masten SJ
FAU - Lockwood, B R
AU  - Lockwood BR
FAU - Trosko, J E
AU  - Trosko JE
LA  - eng
GR  - 2P42ES04911-05/ES/NIEHS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Fundam Appl Toxicol
JT  - Fundamental and applied toxicology : official journal of the Society of 
      Toxicology
JID - 8200838
RN  - 0 (Fluorenes)
RN  - 0 (Polycyclic Compounds)
RN  - 0 (Pyrenes)
RN  - 3417WMA06D (Benzo(a)pyrene)
RN  - 360UOL779Z (fluoranthene)
RN  - 66H7ZZK23N (Ozone)
RN  - 9E0T7WFW93 (pyrene)
SB  - IM
MH  - Animals
MH  - Benzo(a)pyrene/toxicity
MH  - Cell Communication/*drug effects
MH  - Cells, Cultured
MH  - Epithelium/drug effects
MH  - Fluorenes/toxicity
MH  - Gap Junctions/*drug effects/ultrastructure
MH  - Liver/*drug effects
MH  - Ozone/*toxicity
MH  - Polycyclic Compounds/*toxicity
MH  - Pyrenes/toxicity
MH  - Rats
MH  - Rats, Inbred F344
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - S0272059084711298 [pii]
AID - 10.1006/faat.1994.1129 [doi]
PST - ppublish
SO  - Fundam Appl Toxicol. 1994 Oct;23(3):470-5. doi: 10.1006/faat.1994.1129.