PMID- 7836896
OWN - NLM
STAT- MEDLINE
DCOM- 19950301
LR  - 20190709
IS  - 0022-0795 (Print)
IS  - 0022-0795 (Linking)
VI  - 143
IP  - 3
DP  - 1994 Dec
TI  - 11 beta-Hydroxysteroid dehydrogenase in the rat placenta: developmental changes 
      and the effects of altered glucocorticoid exposure.
PG  - 505-13
AB  - The enzyme 11 beta-hydroxysteroid dehydrogenase (11 beta-HSD) catalyses the 
      interconversion of corticosterone, the major glucocorticoid of the rat, and the 
      biologically-inactive 11-dehydrocorticosterone. In the placenta, 11 beta-HSD is 
      thought to regulate glucocorticoid transport between maternal and fetal 
      compartments, and may also affect the local action of glucocorticoids. The 
      present study assessed whether 11 beta-dehydrogenase (corticosterone to 
      11-dehydrocorticosterone) and 11-oxoreductase (11-dehydrocorticosterone to 
      corticosterone) activities are both present in rat placenta, and whether these 
      activities change with advancing pregnancy. Enzyme activity was estimated on days 
      16, 19 and 22 of pregnancy (term = day 23) in placental fragments incubated for 6 
      h with either [3H]corticosterone or [3H]11-dehydrocorticosterone. The percentage 
      conversion of these substrates to [3H]11-dehydrocorticosterone and 
      [3H]corticosterone, respectively, were determined at the end of the incubation. 
      Both 11-oxoreductase and 11 beta-dehydrogenase activities were clearly evident in 
      placental tissue fragments, and while 11-oxoreductase activity declined with 
      advancing pregnancy (P < 0.01), 11 beta-dehydrogenase activity increased (P < 
      0.01). Thus, 11-oxoreductase exceeded (P < 0.05) 11 beta-dehydrogenase at day 16, 
      but thereafter activities were similar. These changes do not appear to be 
      glucocorticoid-induced, since pretreatment of rats with either metyrapone or 
      dexamethasone acetate from day 15 of pregnancy did not affect placental 11 
      beta-HSD on day 22.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Burton, P J
AU  - Burton PJ
AD  - Department of Anatomy and Human Biology, University of Western Australia, 
      Nedlands, Perth.
FAU - Waddell, B J
AU  - Waddell BJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Endocrinol
JT  - The Journal of endocrinology
JID - 0375363
RN  - 0 (Glucocorticoids)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 1.1.- (Hydroxysteroid Dehydrogenases)
RN  - EC 1.1.1.146 (11-beta-Hydroxysteroid Dehydrogenases)
RN  - FO4V44A3G3 (11-dehydrocorticosterone)
RN  - W980KJ009P (Corticosterone)
RN  - ZS9KD92H6V (Metyrapone)
SB  - IM
MH  - 11-beta-Hydroxysteroid Dehydrogenases
MH  - Animals
MH  - Corticosterone/analogs & derivatives/metabolism
MH  - Dexamethasone/pharmacology
MH  - Female
MH  - Gestational Age
MH  - Glucocorticoids/*metabolism
MH  - Hydroxysteroid Dehydrogenases/*metabolism
MH  - Metyrapone/pharmacology
MH  - Placenta/drug effects/*enzymology
MH  - Pregnancy
MH  - Rats
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - 10.1677/joe.0.1430505 [doi]
PST - ppublish
SO  - J Endocrinol. 1994 Dec;143(3):505-13. doi: 10.1677/joe.0.1430505.