PMID- 7840975
OWN - NLM
STAT- MEDLINE
DCOM- 19950309
LR  - 20061115
IS  - 0736-6205 (Print)
IS  - 0736-6205 (Linking)
VI  - 17
IP  - 5
DP  - 1994 Nov
TI  - High resolution mapping using fluorescence in situ hybridization to extended DNA 
      fibers prepared from agarose-embedded cells.
PG  - 928-9, 932-3
AB  - Fluorescence in situ hybridization (FISH) and pulse field gel electrophoresis 
      (PFGE) are essential techniques in physical mapping and in positional cloning. We 
      present a technique that utilizes agarose-embedded high molecular weight DNA 
      prepared for PFGE as a target for FISH. The agarose blocks are melted, and the 
      DNA is extended on a poly-L-lysine-coated microscope slide. The resulting DNA 
      fibers appear on the slide as long straight strands and are a suitable target for 
      high resolution FISH mapping as demonstrated here with cosmid and plasmid 
      hybridizations.
FAU - Heiskanen, M
AU  - Heiskanen M
AD  - University of Helsinki, Finland.
FAU - Karhu, R
AU  - Karhu R
FAU - Hellsten, E
AU  - Hellsten E
FAU - Peltonen, L
AU  - Peltonen L
FAU - Kallioniemi, O P
AU  - Kallioniemi OP
FAU - Palotie, A
AU  - Palotie A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Biotechniques
JT  - BioTechniques
JID - 8306785
RN  - 25104-18-1 (Polylysine)
RN  - 9007-49-2 (DNA)
RN  - 9012-36-6 (Sepharose)
SB  - IM
MH  - Chromosome Mapping/*methods
MH  - Cosmids
MH  - DNA/*metabolism
MH  - Electrophoresis, Gel, Pulsed-Field
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Lymphocytes/chemistry
MH  - Microscopy, Fluorescence
MH  - Plasmids
MH  - Polylysine
MH  - Restriction Mapping
MH  - *Sepharose
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
PST - ppublish
SO  - Biotechniques. 1994 Nov;17(5):928-9, 932-3.