PMID- 7841354
OWN - NLM
STAT- MEDLINE
DCOM- 19950307
LR  - 20041117
IS  - 1148-5493 (Print)
IS  - 1148-5493 (Linking)
VI  - 5
IP  - 4
DP  - 1994 Jul-Aug
TI  - Cytokine expression in intestinal mucosal biopsies. In situ hybridisation of the 
      mRNA for interleukin-1 beta, interleukin-6 and tumour necrosis factor-alpha in 
      inflammatory bowel disease.
PG  - 387-95
AB  - Etiology and pathogenesis of inflammatory bowel disease (IBD) remain obscure. 
      There is substantial evidence that proinflammatory cytokines such as 
      Interleukin-1 beta (IL-1 beta), Interleukin-6 (IL-6) and Tumour Necrosis 
      Factor-alpha (TNF-alpha) exhibit a key role in the the inflammatory process. In 
      situ hybridisation can depict individual cells producing cytokine mRNA. We 
      performed hybridisation with antisense probes specific for IL-1 beta, IL-6 and 
      TNF-alpha on sections of paraffine-embedded biopsies. Specimens obtained from 
      three control persons and six cases of Crohn's disease (CD) were investigated. 
      Only few positive cells were found in tissue sections of control persons, 
      clusters of lamina propria cells or epithelial cells containing cytokine mRNA 
      were not observed. Inflammatory bowel disease tissue contained large numbers of 
      cells producing mRNA specific for the three proinflammatory cytokines assayed. 
      IL-1 beta, IL-6 and TNF-alpha mRNA were predominantly detected corresponding to 
      cells of the lamina propria. Single cells containing mRNA specific for IL-6 were 
      also found among the epithelial lining of intestinal crypts. Epithelial cells 
      containing IL-1 beta and IL-6 mRNA were found in specimens derived from one 
      patient with Crohn's disease. Notably, large amounts of cells containing cytokine 
      mRNA were not only found in inflamed, but also in macroscopically normal mucosa. 
      In conclusion, using proinflammatory cytokines as a model, we established in situ 
      hybridisation on sections of mucosal biopsies permitting further insight into 
      immune activation at individual cell level.
FAU - Woywodt, A
AU  - Woywodt A
AD  - Institute of Immunology and Transfusion Medicine, University of Lubeck Medical 
      School, Germany.
FAU - Neustock, P
AU  - Neustock P
FAU - Kruse, A
AU  - Kruse A
FAU - Schwarting, K
AU  - Schwarting K
FAU - Ludwig, D
AU  - Ludwig D
FAU - Stange, E F
AU  - Stange EF
FAU - Kirchner, H
AU  - Kirchner H
LA  - eng
PT  - Journal Article
PL  - France
TA  - Eur Cytokine Netw
JT  - European cytokine network
JID - 9100879
RN  - 0 (Interleukin-1)
RN  - 0 (Interleukin-6)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - Biopsy
MH  - Crohn Disease/*metabolism/pathology
MH  - Female
MH  - Humans
MH  - In Situ Hybridization
MH  - Interleukin-1/*biosynthesis/genetics
MH  - Interleukin-6/*biosynthesis/genetics
MH  - Intestinal Mucosa/*metabolism/pathology
MH  - Male
MH  - Paraffin Embedding
MH  - RNA, Messenger/*analysis
MH  - Reference Values
MH  - Tumor Necrosis Factor-alpha/*biosynthesis/genetics
EDAT- 1994/07/01 00:00
MHDA- 1994/07/01 00:01
CRDT- 1994/07/01 00:00
PHST- 1994/07/01 00:00 [pubmed]
PHST- 1994/07/01 00:01 [medline]
PHST- 1994/07/01 00:00 [entrez]
PST - ppublish
SO  - Eur Cytokine Netw. 1994 Jul-Aug;5(4):387-95.