PMID- 7841657
OWN - NLM
STAT- MEDLINE
DCOM- 19950309
LR  - 20191023
IS  - 0890-6238 (Print)
IS  - 0890-6238 (Linking)
VI  - 8
IP  - 5
DP  - 1994 Sep-Oct
TI  - Preliminary evidence of phenytoin-induced alterations in embryonic gene 
      expression in a mouse model.
PG  - 383-95
AB  - SWV mouse embryos collected on gestational days (GD) 9:12 and 10:00 following 
      chronic in utero exposure to teratogenic concentrations of phenytoin were 
      utilized for in situ transcription studies of gene expression. The substrate cDNA 
      obtained from the frozen embryo sections was amplified into radiolabelled 
      antisense RNA (RT/aRNA) and used as a probe to screen a panel of 20 cDNA clones 
      representing genes that are important regulators of craniofacial and neural 
      development. The magnitude of alteration in gene expression following phenytoin 
      treatment was determined densitometrically by changes in the hybridization 
      intensity of the aRNA probes to the cDNA clones immobilized to the slot blots. We 
      found that both Wnt-1 and the calcium channel gene were developmentally 
      regulated, as their level of expression decreased significantly between the two 
      collection times. Phenytoin treatment produced a significant downregulation in 
      the level of expression for 25% of the genes examined in the GD 9:12 embryos, 
      including the growth factors TGF-beta and NT3, the proto-oncogene Wnt-1, the 
      nicotinic receptor, and the voltage sensitive calcium channel gene. Additional 
      changes in the coordinate expression of several of the growth and transcription 
      factors were observed at both gestational timepoints. The application of RT/aRNA 
      technology has extended our appreciation of the normal patterns of gene 
      expression during craniofacial and neural development, and provided the first 
      demonstration of multiple coordinate changes in transcription patterns following 
      teratogenic insult.
FAU - Musselman, A C
AU  - Musselman AC
AD  - Department of Veterinary Anatomy and Public Health, College of Veterinary 
      Medicine, Texas A&M University, College Station 77843-4458.
FAU - Bennett, G D
AU  - Bennett GD
FAU - Greer, K A
AU  - Greer KA
FAU - Eberwine, J H
AU  - Eberwine JH
FAU - Finnell, R H
AU  - Finnell RH
LA  - eng
GR  - DE 11303/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Reprod Toxicol
JT  - Reproductive toxicology (Elmsford, N.Y.)
JID - 8803591
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calcium Channels)
RN  - 0 (Ciliary Neurotrophic Factor)
RN  - 0 (DNA, Complementary)
RN  - 0 (Nerve Growth Factors)
RN  - 0 (Nerve Tissue Proteins)
RN  - 0 (Neurotrophin 3)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Antisense)
RN  - 0 (Receptors, Nicotinic)
RN  - 0 (Transforming Growth Factor beta)
RN  - 6158TKW0C5 (Phenytoin)
SB  - IM
GS  - BDNF
GS  - CTNF
GS  - IGF
GS  - NGF
GS  - NT3
GS  - TGF
GS  - Wnt-1
GS  - c-fos
GS  - c-jun
GS  - creb
GS  - trk
MH  - Analysis of Variance
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor
MH  - Calcium Channels/drug effects
MH  - Ciliary Neurotrophic Factor
MH  - Cloning, Molecular
MH  - DNA, Complementary/genetics
MH  - Disease Models, Animal
MH  - Down-Regulation
MH  - Embryonic and Fetal Development/drug effects/genetics
MH  - Female
MH  - Gene Expression Regulation, Developmental/*drug effects/genetics
MH  - Genes
MH  - Gestational Age
MH  - Mice
MH  - Nerve Growth Factors/drug effects/genetics
MH  - Nerve Tissue Proteins/drug effects/genetics
MH  - Neurotrophin 3
MH  - Nucleic Acid Hybridization
MH  - Phenytoin/administration & dosage/*toxicity
MH  - Pregnancy
MH  - Proto-Oncogene Proteins/drug effects/metabolism
MH  - RNA, Antisense/genetics
MH  - Receptors, Nicotinic/drug effects/genetics
MH  - Transcription, Genetic/drug effects/genetics
MH  - Transforming Growth Factor beta/drug effects/genetics
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
AID - 10.1016/0890-6238(94)90078-7 [doi]
PST - ppublish
SO  - Reprod Toxicol. 1994 Sep-Oct;8(5):383-95. doi: 10.1016/0890-6238(94)90078-7.