PMID- 7841659
OWN - NLM
STAT- MEDLINE
DCOM- 19950309
LR  - 20191023
IS  - 0890-6238 (Print)
IS  - 0890-6238 (Linking)
VI  - 8
IP  - 5
DP  - 1994 Sep-Oct
TI  - Gamma-glutamyl transferase (GGT) activity and biochemical characterization of rat 
      visceral yolk-sac during gestation with or without trypan blue exposure.
PG  - 405-10
AB  - Yolk-sacs from untreated Sprague-Dawley rat conceptuses were removed on 
      gestational days (GD) 9 to 18 and examined for gamma-glutamyl transferase (GGT), 
      alkaline phosphatase (AP), lactate dehydrogenase (LDH), and glutamic-oxaloacetic 
      transaminase (GOT) activities. All enzyme activities were found to increase 
      through gestation in total activity as well as in specific activity. Protein 
      (PRO) and urea nitrogen (UN) were also found to increase through gestation 
      whereas triglyceride (TRI) increased steadily from GD 9 to 13 and then appeared 
      to plateau through GD 19. Additional rats were treated on GD 8 with 75 mg trypan 
      blue (TB)/kg body weight and yolk-sacs taken on GD 9 to 18. Yolk-sac GGT and GOT 
      activities from TB-treated rats were significantly higher than the respective 
      controls during early gestation but recovered to or were lower than control 
      levels during midgestation. GGT activity in treated rats was significantly higher 
      than the controls on GD 15 and 16, and both GGT and GOT were significantly lower 
      than controls on GD 17 and 18. AP activity in the TB-treated yolk-sacs was 
      significantly lower than that in controls during early and midgestation but was 
      not significantly different from the control values late in gestation. 
      Triglyceride concentration was not affected early in gestation but significantly 
      decreased on GD 16 and 18. Thus, the yolk-sac enzymes monitored, which are 
      associated with nutrition and normal growth, increased in activity through GD 18. 
      The yolk-sac toxicant, trypan blue, significantly affected enzymatic activity at 
      various time periods during gestation and resulted in significant changes in 
      yolk-sac protein and triglyceride content.
FAU - Andrews, J E
AU  - Andrews JE
AD  - Developmental Toxicology Division, United States Environmental Protection Agency, 
      Research Triangle Park, NC 27711.
FAU - Ebron-McCoy, M
AU  - Ebron-McCoy M
FAU - Logsdon, T R
AU  - Logsdon TR
FAU - Richards, J
AU  - Richards J
FAU - Rogers, J M
AU  - Rogers JM
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Reprod Toxicol
JT  - Reproductive toxicology (Elmsford, N.Y.)
JID - 8803591
RN  - 0 (Pregnancy Proteins)
RN  - 0 (Triglycerides)
RN  - 8W8T17847W (Urea)
RN  - EC 1.1.1.14 (L-Iditol 2-Dehydrogenase)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 2.3.2.2 (gamma-Glutamyltransferase)
RN  - EC 2.6.1.1 (Aspartate Aminotransferases)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
RN  - I2ZWO3LS3M (Trypan Blue)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Analysis of Variance
MH  - Animals
MH  - Aspartate Aminotransferases/metabolism
MH  - Embryonic and Fetal Development/drug effects
MH  - Female
MH  - Gestational Age
MH  - L-Iditol 2-Dehydrogenase/metabolism
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Maternal-Fetal Exchange/drug effects
MH  - Pregnancy
MH  - Pregnancy Proteins/drug effects/metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Triglycerides/metabolism
MH  - Trypan Blue/administration & dosage/*toxicity
MH  - Urea/analysis
MH  - Yolk Sac/drug effects/*enzymology
MH  - gamma-Glutamyltransferase/*metabolism
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
AID - 10.1016/0890-6238(94)90080-9 [doi]
PST - ppublish
SO  - Reprod Toxicol. 1994 Sep-Oct;8(5):405-10. doi: 10.1016/0890-6238(94)90080-9.