PMID- 7842167
OWN - NLM
STAT- MEDLINE
DCOM- 19950307
LR  - 20191111
IS  - 1071-2690 (Print)
IS  - 1071-2690 (Linking)
VI  - 30A
IP  - 10
DP  - 1994 Oct
TI  - Regulation of TGF beta 3 gene expression in embryonic palatal tissue.
PG  - 671-9
AB  - The TGF beta family of genes has been shown to play an important role in 
      regulating various aspects of development, although the mechanisms by which TGF 
      beta exerts its effects have not yet been clarified. Growth and differentiation 
      of both murine embryonic palate mesenchymal (MEPM) cells and palatal epithelium 
      can be regulated by the TGF beta s. We therefore examined the expression of mRNAs 
      encoding TGF beta 1, TGF beta 2, and TGF beta 3 in developing embryonic palatal 
      tissue as well as factors that modulate their levels of expression. Northern blot 
      analysis of RNA isolated from murine embryonic palatal tissue on gestational days 
      (GD) 12, 13, and 14 demonstrated the presence of one mRNA transcript for TGF beta 
      1 (2.5 kb), two transcripts for TGF beta 2 (4.4 kb, 6.0 kb), and one transcript 
      for TGF beta 3 (3.5 kb). Although steady-state levels of TGF beta 1 mRNA showed 
      no changes during development of the palate, TGF beta 2 mRNA levels were maximal 
      on both GD13 and GD14 and TGF beta 3 mRNA levels transiently increased on GD 13. 
      In addition, levels of TGF beta 3 mRNA seemed much higher than either TGF beta 1 
      or TGF beta 2. both TGF beta 1 and TGF beta 2 were able to increase, in a 
      dose-related manner, the expression of TGF beta 3 mRNA in murine embryonic palate 
      mesenchymal cells in vitro. In contrast, epidermal growth factor (EGF) 
      down-regulated the expression of TGF beta 3 mRNA even in the presence of TGF beta 
      1 or TGF beta 2.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Gehris, A L
AU  - Gehris AL
AD  - Department of Anatomy and Developmental Biology, Jefferson Medical College, 
      Thomas Jefferson University, Philadelphia, Pennsylvania 19107.
FAU - Pisano, M M
AU  - Pisano MM
FAU - Nugent, P
AU  - Nugent P
FAU - Greene, R M
AU  - Greene RM
LA  - eng
GR  - DE05550/DE/NIDCR NIH HHS/United States
GR  - DE08199/DE/NIDCR NIH HHS/United States
GR  - DE09540/DE/NIDCR NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - In Vitro Cell Dev Biol Anim
JT  - In vitro cellular & developmental biology. Animal
JID - 9418515
RN  - 0 (Culture Media)
RN  - 0 (RNA, Messenger)
RN  - 0 (Transforming Growth Factor beta)
RN  - 5688UTC01R (Tretinoin)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - E0399OZS9N (Cyclic AMP)
SB  - IM
GS  - TGF&Bgr;
MH  - Animals
MH  - Blotting, Northern
MH  - Cell Division
MH  - Cells, Cultured
MH  - Culture Media
MH  - Cyclic AMP/pharmacology
MH  - Epidermal Growth Factor/pharmacology
MH  - Female
MH  - *Gene Expression Regulation/drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred ICR
MH  - Palate/*embryology/*metabolism
MH  - RNA, Messenger/metabolism
MH  - Transforming Growth Factor beta/*genetics
MH  - Tretinoin/pharmacology
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - 10.1007/BF02631270 [doi]
PST - ppublish
SO  - In Vitro Cell Dev Biol Anim. 1994 Oct;30A(10):671-9. doi: 10.1007/BF02631270.