PMID- 78440
OWN - NLM
STAT- MEDLINE
DCOM- 19780828
LR  - 20061115
IS  - 0026-8984 (Print)
IS  - 0026-8984 (Linking)
VI  - 12
IP  - 3
DP  - 1978 May-Jun
TI  - [Relative positions of stainable portions of DNA in deoxyribonucleoprotein 
      fibrils].
PG  - 663-8
AB  - The effect of acridine orange (AO) concentration on fluorescence polarization of 
      DNA, DNP100 and DNP250 solutions (100 A and 249 A thick DNP fibers, respectively) 
      was studied. It was shown that fluorescence depolarization by an excitation 
      energy migration mechanisms is 3.5 times more efficient for DNP250 than for 
      DNP100. Higher efficiency of energy migration may be explained by a tighter 
      packing of stained DNA regions inside the 250 A fiber because both DNP100 and 
      DNP250 have equal numbers of AO binding sites. The calculated average number of 
      packed DNA strands in DNP250 is 3, the average angle between them is 26.5 
      degrees. A model of the 250 A DNP fiber is proposed. In this model nucleosomes 
      form a tight 250 A coil with a 430 A pitch, internucleosomal DNA strands are 
      oriented approximately along the fiber axis.
FAU - Kishchenko, G P
AU  - Kishchenko GP
FAU - Gruzdev, A D
AU  - Gruzdev AD
LA  - rus
PT  - English Abstract
PT  - Journal Article
TT  - Vzaimnoe raspolozhenie okrashivaemykh uchastkov DNK v fibrillakh 
      dezoksiribonukleoproteidov.
PL  - Russia (Federation)
TA  - Mol Biol (Mosk)
JT  - Molekuliarnaia biologiia
JID - 0105454
RN  - 0 (Acridines)
RN  - 0 (Deoxyribonucleoproteins)
RN  - 0 (Nucleoproteins)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Acridines
MH  - Binding Sites
MH  - *DNA
MH  - *Deoxyribonucleoproteins
MH  - Nucleic Acid Conformation
MH  - *Nucleoproteins
MH  - Protein Conformation
MH  - Staining and Labeling
EDAT- 1978/05/01 00:00
MHDA- 1978/05/01 00:01
CRDT- 1978/05/01 00:00
PHST- 1978/05/01 00:00 [pubmed]
PHST- 1978/05/01 00:01 [medline]
PHST- 1978/05/01 00:00 [entrez]
PST - ppublish
SO  - Mol Biol (Mosk). 1978 May-Jun;12(3):663-8.