PMID- 7844362
OWN - NLM
STAT- MEDLINE
DCOM- 19950309
LR  - 20190512
IS  - 0022-1899 (Print)
IS  - 0022-1899 (Linking)
VI  - 171
IP  - 2
DP  - 1995 Feb
TI  - Detection of human herpesvirus 6 in plasma of children with primary infection and 
      immunosuppressed patients by polymerase chain reaction.
PG  - 273-80
AB  - A sensitive and specific polymerase chain reaction method for the detection of 
      human herpesvirus 6 (HHV-6) DNA in serum or plasma has been developed. In total, 
      157 human serum or plasma samples were studied. HHV-6 DNA was detected in 6 
      (85.7%) of 7 children with exanthem subitum, 3 (23.1%) of 13 bone marrow 
      transplant (BMT) recipients, 4 (22.2%) of 18 human immunodeficiency virus 
      (HIV)-infected patients, 1 (2.6%) of 39 patients with chronic fatigue syndrome, 
      and none of 37 healthy adults. In the HHV-6-positive BMT recipients, HHV-6 plasma 
      DNA was transiently detected during episodes of fever and respiratory infection. 
      In children with exanthem subitum and in 1 HIV-infected patient, the HHV-6 
      strains were characterized as variant B, whereas variant A was detected in all 
      other patients. Detection of viral DNA in serum or plasma is a marker of active 
      infection that can be used to investigate the role of HHV-6 in human disease.
FAU - Secchiero, P
AU  - Secchiero P
AD  - Laboratory of Tumor Cell Biology, National Cancer Institute, National Institutes 
      of Health, Bethesda, Maryland 20892.
FAU - Carrigan, D R
AU  - Carrigan DR
FAU - Asano, Y
AU  - Asano Y
FAU - Benedetti, L
AU  - Benedetti L
FAU - Crowley, R W
AU  - Crowley RW
FAU - Komaroff, A L
AU  - Komaroff AL
FAU - Gallo, R C
AU  - Gallo RC
FAU - Lusso, P
AU  - Lusso P
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Infect Dis
JT  - The Journal of infectious diseases
JID - 0413675
RN  - 0 (DNA, Viral)
SB  - IM
MH  - Adult
MH  - Base Sequence
MH  - Bone Marrow Transplantation
MH  - Child
MH  - Child, Preschool
MH  - DNA, Viral/*blood/genetics
MH  - Exanthema Subitum/blood/complications/*diagnosis
MH  - Female
MH  - Genetic Variation
MH  - HIV Infections/complications
MH  - Herpesviridae Infections/blood/complications/*diagnosis
MH  - Herpesvirus 6, Human/genetics/*isolation & purification
MH  - Humans
MH  - *Immunocompromised Host
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Polymerase Chain Reaction/*methods
MH  - Sensitivity and Specificity
MH  - Viremia/diagnosis
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
AID - 10.1093/infdis/171.2.273 [doi]
PST - ppublish
SO  - J Infect Dis. 1995 Feb;171(2):273-80. doi: 10.1093/infdis/171.2.273.