PMID- 7844567
OWN - NLM
STAT- MEDLINE
DCOM- 19950307
LR  - 20090929
IS  - 0022-1317 (Print)
IS  - 0022-1317 (Linking)
VI  - 76 ( Pt 2)
DP  - 1995 Feb
TI  - Characterization of human telomeric repeat sequences from human herpesvirus 6 and 
      relationship to replication.
PG  - 451-8
AB  - Here we examine by polymerase chain reaction amplification followed by cloning 
      and sequence analyses selected regions of the human herpesvirus 6 (HHV-6) genome 
      which contain human telomeric repeats (TTA-GGG). We determine the relative 
      number, arrangement and orientation of the repeats in the unit length genome, in 
      concatemeric replicative intermediates and in heterogeneous (het) regions. We 
      also examine distribution of the repeats in the entire genome (159 kb) and their 
      orientation relative to DNA packaging motifs and the origin of lytic replication. 
      In the prototype orientation the HHV-6 repeat is the related complement, TAACCC. 
      We show that tandem arrays of this repeat are present in the right and left long 
      direct repeats (DRL and DRR, 8 kb each) which bound the long unique sequence (UL, 
      143 kb). Within each DR there is a left terminal imperfect tandem array and a 
      right terminal perfect tandem array (58 copies). In DR they are each adjacent to 
      DNA packaging motifs, pac1 and pac2, described for herpes simplex virus and human 
      cytomegalovirus, in the arrangement pac1-imperfect repeat-7.2 kb-perfect 
      repeat-pac2. Five independent clones were isolated and sequence determined from 
      junctions of concatemeric replicative intermediates which showed adjacent pac2 
      and pac1 motifs surrounded by telomeric repeats. Favoured cleavage sites for unit 
      length genomes were indicated which avoided cleavage within the repeats. Analyses 
      of the complete genome showed no tandem repeats within UL but did show a polar 
      distribution of monomeric copies and related sequences around the origin of 
      replication, with an effect on the overall base composition. The implications for 
      virus replication are discussed.
FAU - Gompels, U A
AU  - Gompels UA
AD  - Department of Clinical Sciences, London School of Hygiene and Tropical Medicine, 
      University of London, UK.
FAU - Macaulay, H A
AU  - Macaulay HA
LA  - eng
SI  - GENBANK/X79798
SI  - GENBANK/X79799
SI  - GENBANK/X79800
GR  - Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Gen Virol
JT  - The Journal of general virology
JID - 0077340
SB  - IM
MH  - Amino Acid Sequence
MH  - Base Sequence
MH  - Genome, Viral
MH  - Herpesvirus 6, Human/*genetics/physiology
MH  - Molecular Sequence Data
MH  - *Repetitive Sequences, Nucleic Acid
MH  - *Telomere
MH  - *Virus Replication
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
AID - 10.1099/0022-1317-76-2-451 [doi]
PST - ppublish
SO  - J Gen Virol. 1995 Feb;76 ( Pt 2):451-8. doi: 10.1099/0022-1317-76-2-451.