PMID- 7846626
OWN - NLM
STAT- MEDLINE
DCOM- 19950306
LR  - 20190913
IS  - 0039-6060 (Print)
IS  - 0039-6060 (Linking)
VI  - 117
IP  - 2
DP  - 1995 Feb
TI  - Induction of acute pancreatitis in germ-free rats: evidence of a primary role for 
      tumor necrosis factor-alpha.
PG  - 201-5
AB  - BACKGROUND: Tumor necrosis factor-alpha (TNF-alpha) has been implicated as a 
      mediator of the systemic manifestations associated with acute pancreatitis. The 
      purpose of this study was to show that TNF-alpha expression in pancreatitis is a 
      primary response and is not the result of endotoxemia. METHODS: Severe acute 
      pancreatitis was induced in germ-free rats, which have no source of endogenous 
      endotoxin, by ductal infusion of artificial bile. Control animals underwent sham 
      operation and ductal infusion of saline solution. TNF-alpha levels were measured 
      by the WEHI bioassay. Endotoxin was measured by the Limulus assay. RESULTS: 
      TNF-alpha levels remained low in the sham group (mean, 24.6 +/- 8.0 pg/ml) but 
      were significantly elevated in normal rats with pancreatitis (181 +/- 26.8 pg/ml; 
      p < 0.001 versus sham group) and in germ-free rats with pancreatitis (213 +/- 90 
      pg/ml; p < 0.002 versus sham group). No endotoxin was detected in any of the 
      experimental rats. CONCLUSIONS: Our results indicate that TNF-alpha levels are 
      elevated in acute pancreatitis despite the absence of endotoxin, indicating a 
      primary role of TNF-alpha in this disease.
FAU - Hughes, C B
AU  - Hughes CB
AD  - Department of Surgery, University of Tennessee, Memphis 38163.
FAU - Gaber, L W
AU  - Gaber LW
FAU - Kotb, M
AU  - Kotb M
FAU - Mohey el-Din, A B
AU  - Mohey el-Din AB
FAU - Pabst, M
AU  - Pabst M
FAU - Gaber, A O
AU  - Gaber AO
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Surgery
JT  - Surgery
JID - 0417347
RN  - 0 (Endotoxins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 5E090O0G3Z (Taurocholic Acid)
RN  - EC 3.4.21.4 (Trypsin)
SB  - IM
MH  - Acute Disease
MH  - Animals
MH  - Bile
MH  - Endotoxins/analysis
MH  - *Germ-Free Life
MH  - Limulus Test
MH  - Male
MH  - Pancreas/pathology
MH  - Pancreatitis/*etiology/microbiology/pathology
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Taurocholic Acid
MH  - Trypsin
MH  - Tumor Necrosis Factor-alpha/*physiology
EDAT- 1995/02/01 00:00
MHDA- 1995/02/01 00:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 1995/02/01 00:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
AID - S0039-6060(05)80086-8 [pii]
AID - 10.1016/s0039-6060(05)80086-8 [doi]
PST - ppublish
SO  - Surgery. 1995 Feb;117(2):201-5. doi: 10.1016/s0039-6060(05)80086-8.