PMID- 7847100
OWN - NLM
STAT- MEDLINE
DCOM- 19950307
LR  - 20191101
IS  - 0951-3590 (Print)
IS  - 0951-3590 (Linking)
VI  - 8
IP  - 3
DP  - 1994 Sep
TI  - Immunohistochemical localization of interferon-gamma in normal human ovary.
PG  - 161-8
AB  - There is increasing evidence that cytokines are important intraovarian 
      non-steroidal regulators. The aim of the present study was to evaluate the 
      presence in the human ovary of interferon (IFN)-gamma, a cytokine produced by T 
      lymphocytes after mitogenic or antigenic stimulation. Very low levels of 
      IFN-gamma (0.025-0.057 IU/ml) were found in follicular fluid of large 
      spontaneously maturing follicles, in the ovarian vein (< 0.01-0.079 IU/ml) or 
      peripheral blood (< 0.01-0.06 IU/ml). The avidin-biotin immunocytochemical 
      technique, with appropriate monoclonal antibodies, was used to localize 
      IFN-gamma-positive cells, human leukocyte antigen (HLA)-DR molecules, activated T 
      cells, T helper/inducer cells and T cytotoxic/suppressor cells. 
      IFN-gamma-positive cells were only detected in preovulatory follicles, associated 
      with the follicular basal lamina, thecal vessels and interstitial tissue. In the 
      same large follicles very few T helper/inducer cells were detected, but a high 
      proportion of T lymphocytes expressed the CD8 phenotype in the theca, 
      interstitial tissue and follicular cavity. No IFN-gamma-positive cells were 
      observed in preantral and small antral follicles. The results indicate that the 
      human ovary contains immunoreactive IFN-gamma, suggesting that the cytokine plays 
      a paracrine role in human ovarian function.
FAU - Grasso, G
AU  - Grasso G
AD  - Institute of Human Anatomy, Italian Neuroimmunology Group, University of Siena.
FAU - Asano, A
AU  - Asano A
FAU - Minagawa, T
AU  - Minagawa T
FAU - Tanaka, T
AU  - Tanaka T
FAU - Fujimoto, S
AU  - Fujimoto S
FAU - Muscettola, M
AU  - Muscettola M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Gynecol Endocrinol
JT  - Gynecological endocrinology : the official journal of the International Society 
      of Gynecological Endocrinology
JID - 8807913
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (CD8 Antigens)
RN  - 0 (HLA-DR Antigens)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adult
MH  - Antibodies, Monoclonal/analysis/immunology
MH  - CD8 Antigens/analysis
MH  - Female
MH  - Follicular Fluid/chemistry
MH  - HLA-DR Antigens/analysis
MH  - Humans
MH  - Immunohistochemistry
MH  - Interferon-gamma/*analysis/immunology/physiology
MH  - Ovary/*chemistry/cytology/physiology
MH  - Phenotype
MH  - T-Lymphocytes, Helper-Inducer/cytology/physiology
MH  - T-Lymphocytes, Regulatory/physiology
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
AID - 10.3109/09513599409072450 [doi]
PST - ppublish
SO  - Gynecol Endocrinol. 1994 Sep;8(3):161-8. doi: 10.3109/09513599409072450.