PMID- 7847806
OWN - NLM
STAT- MEDLINE
DCOM- 19950309
LR  - 20071114
IS  - 0250-7005 (Print)
IS  - 0250-7005 (Linking)
VI  - 14
IP  - 5A
DP  - 1994 Sep-Oct
TI  - Rearrangement of chromosome band 11q13 in HeLa cells.
PG  - 1727-33
AB  - Previous somatic cell genetic and molecular genetic studies have localized the 
      Hela cell tumor suppressor gene to the long arm of chromosome 11. To determine if 
      loss of heterozygosity has occurred for the inactivation of the tumor suppressor 
      gene, we investigated the molecular status of chromosome 11 in the HeLa cells. 
      The restriction fragment length polymorphic (RFLP) analysis of four different 
      HeLa sublines showed retention of heterozygosity for the long and short arm 
      specific probes in three of the sublines. Fluorescence in situ hybridization 
      (FISH) studies using whole chromosome painting probes identified 11 material in a 
      marker chromosome in addition to the two normal appearing 11's in these three 
      cell lines. The fourth cell line contained only two 11's. This cell line had lost 
      the 11 material from the marker chromosome. Both molecular and karyotypic 
      analyses indicated q13 as the common site of rearrangement in the derivation of 
      these marker chromosomes. We therefore conclude that 11q13 rearrangement could 
      contribute to the abnormal growth behaviour of HeLa cells in vivo and/or in 
      vitro.
FAU - Jesudasan, R A
AU  - Jesudasan RA
AD  - Department of Surgery, UCLA School of Medicine, VA Medical Center, Wadsworth 
      Division 90073.
FAU - Slovak, M L
AU  - Slovak ML
FAU - Sen, S
AU  - Sen S
FAU - Srivatsan, E S
AU  - Srivatsan ES
LA  - eng
GR  - CA33572/CA/NCI NIH HHS/United States
GR  - CA47206/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Genetic Markers)
SB  - IM
MH  - Alleles
MH  - Chromosome Banding
MH  - *Chromosomes, Human, Pair 11
MH  - DNA Probes
MH  - DNA, Neoplasm/genetics
MH  - *Gene Rearrangement
MH  - Genetic Markers
MH  - HeLa Cells/physiology/*ultrastructure
MH  - Heterozygote
MH  - Humans
MH  - Polymorphism, Genetic
MH  - Polymorphism, Restriction Fragment Length
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
PST - ppublish
SO  - Anticancer Res. 1994 Sep-Oct;14(5A):1727-33.