PMID- 7848833
OWN - NLM
STAT- MEDLINE
DCOM- 19950316
LR  - 20181130
IS  - 0214-6282 (Print)
IS  - 0214-6282 (Linking)
VI  - 38
IP  - 3
DP  - 1994 Sep
TI  - Expression of transforming growth factor alpha (TGF alpha), epidermal growth 
      factor receptor (EGF-R) and cell proliferation during human palatogenesis: an 
      immunohistochemical study.
PG  - 499-505
AB  - Transforming growth factor alpha (TGF alpha) is not only a potent mitogen for 
      several cell types, it interferes with cell differentiation. To investigate the 
      possible role of TGF alpha in the fusion of the palatal processes in humans, the 
      distribution of TGF alpha and its receptor (epidermal growth factor receptor = 
      EGF-R) were studied using immunohistochemistry. 23 human palates were obtained 
      from embryos and fetuses at 6 to 12 weeks of gestation and embedded in paraffin. 
      In each case, the degree of cell proliferation was assessed using an antibody 
      reacting with the nuclear antigen Ki-67. The epithelial and mesenchymal cell 
      phenotypes were studied with anti-cytokeratin and anti-vimentin antibodies. TGF 
      alpha and its receptor were detected in all the human palates, regardless of the 
      stage of fusion. They were more highly expressed in the epithelial cells than in 
      the mesenchymal cells of the palatal shelves. At first, proliferative activity 
      was intense in both the mesenchyme and the epithelia and was later principally 
      limited to the nasal or oral epithelia and also to the degenerating epithelial 
      seam. At 10 weeks, when the midline palatal seam broke up into epithelial 
      islands, the epithelial cells remained immunolabeled for TGF alpha, EGF-R and 
      showed an increased number of proliferating cells. Programmed cell death (PCD) of 
      medial edge epithelia (MEE) has been well documented, however other mechanisms 
      must be considered during palatogenesis. Complex interactions between different 
      growth factors have a probable role in epithelial mesenchymal transformation 
      (EMT) and migration as well as in extracellular matrix synthesis.
FAU - Citterio, H L
AU  - Citterio HL
AD  - Laboratoire Pol Bouin, CHU Reims, INSERM U 314, France.
FAU - Gaillard, D A
AU  - Gaillard DA
LA  - eng
PT  - Journal Article
PL  - Spain
TA  - Int J Dev Biol
JT  - The International journal of developmental biology
JID - 8917470
RN  - 0 (Transforming Growth Factor alpha)
RN  - EC 2.7.10.1 (ErbB Receptors)
SB  - IM
MH  - Cell Differentiation
MH  - Cell Division
MH  - Epithelial Cells
MH  - ErbB Receptors/*analysis
MH  - Humans
MH  - *Maxillofacial Development
MH  - Palate/chemistry/cytology/*embryology
MH  - Transforming Growth Factor alpha/*analysis
EDAT- 1994/09/01 00:00
MHDA- 1994/09/01 00:01
CRDT- 1994/09/01 00:00
PHST- 1994/09/01 00:00 [pubmed]
PHST- 1994/09/01 00:01 [medline]
PHST- 1994/09/01 00:00 [entrez]
PST - ppublish
SO  - Int J Dev Biol. 1994 Sep;38(3):499-505.