PMID- 7850744
OWN - NLM
STAT- MEDLINE
DCOM- 19950316
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 79
IP  - 1
DP  - 1995 Jan
TI  - Identification of the chromosome 12 translocation breakpoint region of a 
      pleomorphic salivary gland adenoma with t(1;12)(p22;q15) as the sole cytogenetic 
      abnormality.
PG  - 1-7
AB  - Cell line Ad-312/SV40, which was derived from a primary pleomorphic salivary 
      gland adenoma with t(1;12)(p22;q15), was used in fluorescence in situ 
      hybridization (FISH) analysis to characterize its translocation breakpoint region 
      on chromosome 12. Results of previous studies have indicated that the chromosome 
      12 breakpoint in Ad-312/SV40 is located proximally to locus D12S8 and distally to 
      the CHOP gene. We here describe two partially overlapping yeast artificial 
      chromosome (YAC) clones, Y4854 (500 kbp) and Y9091 (460 kbp), which we isolated 
      in the context of a chromosome walking project with D12S8 and CHOP as starting 
      points. We present a composite long-range restriction map encompassing the 
      inserts of these two YAC clones and show by FISH analysis that both YACs span the 
      chromosome 12 breakpoint as present in Ad-312/SV40 cells. Subsequently, we have 
      isolated cosmid clones corresponding to various sequence-tagged sites (STSs) 
      mapping within the inserts of these YAC clones. These included cRM51, cRM69, 
      cRM85, cRM90, cRM91, cRM110, and cRM111. In FISH studies, cosmid clones cRM85, 
      cRM90, and cRM111 appeared to map distally to the chromosome 12 breakpoint, 
      whereas cosmid clones cRM51, cRM69, cRM91, and cRM110 were found to map 
      proximally to it. These results assign the chromosome 12 breakpoint in 
      Ad-312/SV40 to a DNA region of less than 165 kbp. FISH evaluation of the 
      chromosome 12 breakpoints in five other pleomorphic salivary gland adenoma cell 
      lines indicated that these are located proximally to the one in Ad-312/SV40, at a 
      distance of more than 0.9 Mbp from STS RM91. These results, while pinpointing a 
      potentially critical region on chromosome 12, also provide evidence for the 
      possible involvement of 12q13-q15 sequences located elsewhere.
FAU - Kools, P F
AU  - Kools PF
AD  - Center for Human Genetics, University of Leuven, Belgium.
FAU - Wanschura, S
AU  - Wanschura S
FAU - Schoenmakers, E F
AU  - Schoenmakers EF
FAU - Geurts, J M
AU  - Geurts JM
FAU - Mols, R
AU  - Mols R
FAU - Kazmierczak, B
AU  - Kazmierczak B
FAU - Bullerdiek, J
AU  - Bullerdiek J
FAU - Van den Berghe, H
AU  - Van den Berghe H
FAU - Van de Ven, W J
AU  - Van de Ven WJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adenoma, Pleomorphic/*genetics
MH  - Chromosome Mapping
MH  - *Chromosomes, Human, Pair 1
MH  - *Chromosomes, Human, Pair 12
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Salivary Gland Neoplasms/*genetics
MH  - *Translocation, Genetic
MH  - Tumor Cells, Cultured
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 016546089400137Z [pii]
AID - 10.1016/0165-4608(94)00137-z [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1995 Jan;79(1):1-7. doi: 10.1016/0165-4608(94)00137-z.