PMID- 7850758
OWN - NLM
STAT- MEDLINE
DCOM- 19950316
LR  - 20190816
IS  - 0165-4608 (Print)
IS  - 0165-4608 (Linking)
VI  - 79
IP  - 1
DP  - 1995 Jan
TI  - Characterization of chromosome 8 abnormalities by fluorescence in situ 
      hybridization in childhood B-acute lymphoblastic leukemia/non-Hodgkin lymphoma.
PG  - 8-14
AB  - Using fluorescence in situ hybridization (FISH), we studied chromosome 8 
      abnormalities in 30 children with mature B-cell acute lymphoblastic leukemia 
      (B-ALL) or B-cell non-Hodgkin lymphoma (B-NHL). FISH was performed on metaphase 
      spreads and interphase nuclei with a whole chromosome 8 painting probe. Fifteen 
      patients were studied retrospectively after metaphases from the malignant cell 
      specimen had already been G-banded. When interphase nuclei were examined, FISH 
      was able to confirm t(8;14)(q24;q32) in all nine patients positive by previous 
      G-banding. FISH, however, was positive in metaphase spreads from only seven 
      patients. Another 15 patients were included in a prospective study. In six of 
      them (40%), a translocation involving chromosome 8 was shown by a split small 
      segment (8q24-8qter) on interphase nuclei. Analysis of metaphase spreads showed 
      only three positive cases each by FISH or G-banding, respectively, with 
      corresponding results in two patients. By interphase FISH, trisomy of chromosome 
      8 also was detectable. In three patients shown by G-banding to have trisomy, 
      interphase FISH study showed high scores of three chromosome 8 signal positive 
      cells. There was no cross-hybridization to other chromosomes interfering with 
      FISH analysis. FISH analysis on interphase nuclei using a whole chromosome 8 
      hybridization probe will supplement and can be more sensitive than metaphase 
      cytogenetic techniques for detection of chromosome 8 rearrangements in B-ALL/NHL.
FAU - Nishida, K
AU  - Nishida K
AD  - Department of Pediatrics, Hematology and Oncology, Justus-Liebig-University, 
      Giessen, Germany.
FAU - Ritterbach, J
AU  - Ritterbach J
FAU - Repp, R
AU  - Repp R
FAU - Harbott, J
AU  - Harbott J
FAU - Lampert, F
AU  - Lampert F
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Genet Cytogenet
JT  - Cancer genetics and cytogenetics
JID - 7909240
SB  - IM
MH  - Adolescent
MH  - Burkitt Lymphoma/*genetics
MH  - Child
MH  - Child, Preschool
MH  - *Chromosome Aberrations
MH  - *Chromosomes, Human, Pair 8
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Lymphoma, B-Cell/*genetics
MH  - Male
MH  - Precursor Cell Lymphoblastic Leukemia-Lymphoma/*genetics
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 016546089400096T [pii]
AID - 10.1016/0165-4608(94)00096-t [doi]
PST - ppublish
SO  - Cancer Genet Cytogenet. 1995 Jan;79(1):8-14. doi: 10.1016/0165-4608(94)00096-t.