PMID- 7851045
OWN - NLM
STAT- MEDLINE
DCOM- 19950314
LR  - 20061115
IS  - 0722-5091 (Print)
IS  - 0722-5091 (Linking)
VI  - 13
IP  - 6
DP  - 1994 Nov-Dec
TI  - Lobar pilocytic astrocytomas of the cerebral hemispheres: II. 
      Pathobiology--morphogenesis of the eosinophilic granular bodies.
PG  - 306-14
AB  - This study provides new immunocytochemical observations on the so-called 
      eosinophilic granular bodies (EGBs), seen predominantly (but not exclusively) in 
      pilocytic astrocytomas. Using combined immunohistochemical and immunoelectron 
      microscopic approaches on formalin-fixed, paraffin-embedded tissues, we have 
      demonstrated that (1) EGBs exhibit pronounced reactivity with antibodies to 
      serine protease inhibitors alpha-1-antichymotrypsin and alpha-1-antitrypsin; by 
      immunoelectron microscopy, the reaction product is localized either in the form 
      of diffuse floccular densities, or larger conglomerates of amorphous, globular 
      material; (2) an antiserum to ubiquitin-protein conjugates, codistributes in the 
      EGBs at the light microscopic level, while ultrastructurally is either localized 
      in diffuse, finely granular deposits, and/or fragmented filamentous particles; 
      and (3) that a monoclonal antibody to beta-amyloid precursor protein (beta-APP) 
      stains smaller EGBs. The detection of serpin-like and beta-APP-like staining in 
      EGBs may be a reflection of acute phase reactant activity in response to 
      tumor-produced proteases. We postulate that EGBs contain complexes of serpins and 
      hitherto unknown protease(s), which are in turn probably degraded via ubiquitin 
      mediated mechanism(s). Although EGBs typify pilocytic astrocytomas, they may be 
      exceptionally present in malignant astrocytomas, calling for cautious 
      interpretation of their biologic as well as prognostic import.
FAU - Katsetos, C D
AU  - Katsetos CD
AD  - Department of Pathology, Hahnemann University, Philadelphia, Pennsylvania 
      19102-1192.
FAU - Krishna, L
AU  - Krishna L
FAU - Friedberg, E
AU  - Friedberg E
FAU - Reidy, J
AU  - Reidy J
FAU - Karkavelas, G
AU  - Karkavelas G
FAU - Savory, J
AU  - Savory J
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Germany
TA  - Clin Neuropathol
JT  - Clinical neuropathology
JID - 8214420
RN  - 0 (Amyloid beta-Protein Precursor)
RN  - 0 (Ubiquitins)
RN  - 0 (alpha 1-Antichymotrypsin)
RN  - 0 (alpha 1-Antitrypsin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Amyloid beta-Protein Precursor/analysis
MH  - Astrocytoma/chemistry/*ultrastructure
MH  - Brain Neoplasms/chemistry/*ultrastructure
MH  - Child
MH  - Humans
MH  - Middle Aged
MH  - Ubiquitins/analysis
MH  - alpha 1-Antichymotrypsin/analysis
MH  - alpha 1-Antitrypsin/analysis
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
PST - ppublish
SO  - Clin Neuropathol. 1994 Nov-Dec;13(6):306-14.