PMID- 7851161
OWN - NLM
STAT- MEDLINE
DCOM- 19950314
LR  - 20151119
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 17
IP  - 3
DP  - 1994 Nov 1
TI  - Comparative flow cytometric analysis of Ki-67 and proliferating cell nuclear 
      antigen (PCNA) in solid neoplasms.
PG  - 258-65
AB  - The proliferation associated antigens, Ki-67 and proliferating-cell nuclear 
      antigen (PCNA), have been widely used in studies assessing the growth fraction in 
      human malignancies. It remains unclear, however, whether these markers yield 
      similar assessments of proliferative activity in any given neoplasm. In this 
      study, we compared Ki-67 and PCNA expression in 93 malignant solid neoplasms 
      using bivariate flow cytometric analysis of these antigens and DNA content. The 
      growth fractions measured by Ki-67 and PCNA were compared and correlated with 
      acridine orange (AO) analysis and tumor grade. Our results indicate a significant 
      difference between Ki-67 and PCNA values in neoplasms of low and intermediate 
      grade (P = 0.002); Ki-67 values were significantly lower than those obtained by 
      PCNA in this group. No statistical difference between Ki-67 and PCNA values was 
      found in high grade neoplasms (P = 0.38). Analysis of different cell cycle 
      compartments indicates that the observed difference in the positivity of these 
      markers was due to their differential expression in the G0/1 segment of the cell 
      cycle. We conclude that Ki-67 may better reflect the proliferative activity in 
      solid neoplasms than does PCNA.
FAU - Steck, K
AU  - Steck K
AD  - Department of Pathology, University of Texas M. D. Anderson Cancer Center, 
      Houston 77030.
FAU - el-Naggar, A K
AU  - el-Naggar AK
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (DNA, Neoplasm)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (Nuclear Proteins)
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (RNA, Neoplasm)
SB  - IM
MH  - Biomarkers, Tumor/analysis
MH  - Cell Cycle
MH  - DNA, Neoplasm/analysis
MH  - Flow Cytometry
MH  - Humans
MH  - Ki-67 Antigen
MH  - Neoplasm Proteins/*analysis
MH  - Neoplasms/*chemistry/pathology
MH  - Nuclear Proteins/*analysis
MH  - Proliferating Cell Nuclear Antigen/*analysis
MH  - RNA, Neoplasm/analysis
MH  - Tumor Cells, Cultured
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - 10.1002/cyto.990170309 [doi]
PST - ppublish
SO  - Cytometry. 1994 Nov 1;17(3):258-65. doi: 10.1002/cyto.990170309.