PMID- 7853537
OWN - NLM
STAT- MEDLINE
DCOM- 19950313
LR  - 20200724
IS  - 0022-538X (Print)
IS  - 1098-5514 (Electronic)
IS  - 0022-538X (Linking)
VI  - 69
IP  - 3
DP  - 1995 Mar
TI  - Int-6, a highly conserved, widely expressed gene, is mutated by mouse mammary 
      tumor virus in mammary preneoplasia.
PG  - 1932-8
AB  - With a unique mouse mammary tumor model system in which mouse mammary tumor virus 
      (MMTV) insertional mutations can be detected during progression from preneoplasia 
      to frank malignancy, including metastasis, we have discovered a new common 
      integration site (designated Int-6) for MMTV in mouse mammary tumors. MMTV was 
      integrated into Int-6 in a mammary hyperplastic outgrowth line, its tumors and 
      metastases, and two independent mammary tumors arising in unrelated mice. The 
      Int-6 gene is ubiquitously expressed as a 1.4-kb RNA species in adult tissues and 
      is detected beginning at day 8 of embryonic development. The nucleotide sequence 
      of Int-6 is unrelated to any of the known genes in the GenBank database. MMTV 
      integrates within introns of the gene in the opposite transcriptional 
      orientation. In each tumor tested, this results in the expression of a truncated 
      Int-6/long terminal repeat (LTR) chimeric RNA species which is terminated at a 
      cryptic termination signal in the MMTV LTR. Since the nonrearranged Int-6 alleles 
      in these tumors contain no mutations, we favor the conclusion that truncation of 
      the Int-6 gene product either biologically activates its function or represents a 
      dominant-negative mutation.
FAU - Marchetti, A
AU  - Marchetti A
AD  - Laboratory of Tumor Immunology and Biology, National Cancer Institute, Bethesda, 
      Maryland 20892.
FAU - Buttitta, F
AU  - Buttitta F
FAU - Miyazaki, S
AU  - Miyazaki S
FAU - Gallahan, D
AU  - Gallahan D
FAU - Smith, G H
AU  - Smith GH
FAU - Callahan, R
AU  - Callahan R
LA  - eng
SI  - GENBANK/S75221
SI  - GENBANK/S75223
SI  - GENBANK/S75224
PT  - Journal Article
PL  - United States
TA  - J Virol
JT  - Journal of virology
JID - 0113724
RN  - 0 (Eukaryotic Initiation Factor-3)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 0 (RNA, Messenger)
RN  - 0 (RNA, Neoplasm)
SB  - IM
GS  - Int-6
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Cell Transformation, Viral
MH  - Cloning, Molecular
MH  - Eukaryotic Initiation Factor-3
MH  - Gene Expression Regulation, Viral
MH  - Genes
MH  - Mammary Neoplasms, Experimental/genetics
MH  - Mammary Tumor Virus, Mouse/*genetics
MH  - Mice
MH  - Molecular Sequence Data
MH  - Mutagenesis, Insertional
MH  - Mutation
MH  - Precancerous Conditions/*genetics/microbiology
MH  - Proto-Oncogene Proteins/*genetics
MH  - Proviruses/genetics
MH  - RNA, Messenger/genetics
MH  - RNA, Neoplasm/genetics
MH  - Restriction Mapping
MH  - Sequence Homology, Nucleic Acid
MH  - *Virus Integration
PMC - PMC188811
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
PMCR- 1995/03/01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
PHST- 1995/03/01 00:00 [pmc-release]
AID - 10.1128/JVI.69.3.1932-1938.1995 [doi]
PST - ppublish
SO  - J Virol. 1995 Mar;69(3):1932-8. doi: 10.1128/JVI.69.3.1932-1938.1995.