PMID- 7855119
OWN - NLM
STAT- MEDLINE
DCOM- 19950313
LR  - 20200304
IS  - 0932-0113 (Print)
IS  - 0932-0113 (Linking)
VI  - 80
IP  - 7
DP  - 1994
TI  - Acanthamoeba-specific human T-cell clones isolated from healthy individuals.
PG  - 549-53
AB  - T-cell responses to pathogenic free-living amoebae, Acanthamoeba sp., were 
      analyzed in healthy Japanese individuals. Of 20 healthy subjects, 10 (50%) showed 
      significant proliferative responses of peripheral blood mononuclear cells to the 
      soluble amoebic antigens in vitro. The antigens used were not mitogenic, and no 
      evidence of amoebic superantigens was available. We established human T-cell 
      clones reactive to Acanthamoeba, all of which were CD3- and CD4-positive, 
      CD8-negative, and TCR-alpha beta-positive. We isolated two strains of 
      Acanthamoeba from two patients, one from a patient with meningoencephalitis (CSF 
      strain) and the other from a patient with keratitis (K strain). Of 13 clones, 11 
      were reactive to the K-strain as well as to the CSF-strain antigen under human 
      leukocyte antigen (HLA)-DR restriction, whereas the other two were specific for 
      the K-strain antigen. All but one clone tested showed TH1-equivalent functions 
      because these cells produced interferon (IFN)-gamma in response to the amoebic 
      antigen but produced no detectable level of interleukin 4 (IL-4). These results 
      suggest that immunocompetent hosts might have acquired protective immunity 
      mediated by Acanthamoeba-specific T-cells during natural sensitization.
FAU - Tanaka, Y
AU  - Tanaka Y
AD  - Department of Parasitology, Okayama University Medical School, Japan.
FAU - Suguri, S
AU  - Suguri S
FAU - Harada, M
AU  - Harada M
FAU - Hayabara, T
AU  - Hayabara T
FAU - Suzumori, K
AU  - Suzumori K
FAU - Ohta, N
AU  - Ohta N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - Germany
TA  - Parasitol Res
JT  - Parasitology research
JID - 8703571
RN  - 0 (Antigens, Protozoan)
RN  - 0 (HLA-DR Antigens)
RN  - 207137-56-2 (Interleukin-4)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Acanthamoeba/*immunology/pathogenicity
MH  - Adult
MH  - Animals
MH  - Antigens, Protozoan/*immunology
MH  - Clone Cells
MH  - Female
MH  - HLA-DR Antigens/immunology
MH  - Humans
MH  - Immunity, Cellular
MH  - Infant, Newborn
MH  - Interferon-gamma/biosynthesis
MH  - Interleukin-4/biosynthesis
MH  - Japan
MH  - Lymphocyte Activation
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Middle Aged
MH  - T-Lymphocytes/*immunology
MH  - Th1 Cells/immunology
MH  - Virulence
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
AID - 10.1007/BF00933001 [doi]
PST - ppublish
SO  - Parasitol Res. 1994;80(7):549-53. doi: 10.1007/BF00933001.