PMID- 785918
OWN - NLM
STAT- MEDLINE
DCOM- 19761020
LR  - 20061115
IS  - 0001-5547 (Print)
IS  - 0001-5547 (Linking)
VI  - 20
IP  - 4
DP  - 1976 Jul-Aug
TI  - Comparison of buccal and nasal epithelial cells using a new cell development 
      index and quantitative interference microscopy.
PG  - 372-4
AB  - Cell area, cell dry mass, and three summary indices for tissue maturation were 
      obtained for human buccal and nasal cells viewed in smears (five donors). The 
      means for cell area and dry mass in the buccal smears were 3816 X 10(-8) cm2 and 
      2294 pg (1 pg=1 X 10(-12) g), respectively. The correlation coefficient for means 
      of cell area and dry mass was r=0.03. The means for the three maturation indices 
      used to characterize the cells viewed in buccal smears were: Cell Development 
      Index (CDI), 486; Maturation Index (MI) 50.6; and Karyopyknotic Index (KPI), 
      0.028. The correlation coefficients for means were: CDI vs. MI, r=0.88; CDI vs. 
      KPI, r=0.71; and MI vs. KPI, r=0.73. In the buccal smears, the cell types found 
      most frequently were intermediate with round or oval nuclei, which altogether 
      averaged about 90% of the total smear composition, while superficial cells 
      (pyknotic ghost, and anucleate) comprised less than four per cent. In nasal 
      smears, the means for cell area and dry mass were 867 X 10(-8) cm2 and 623 pg, 
      respectively. The correlation coefficient for means of cell area and dry mass was 
      r=0.74. The means for the maturation indices were: CDI, 816; MI, 91.2; and KPI, 
      0.79. The correlation coefficients for means were CDI vs. MI, r=0.93; CDI vs. 
      KPI, r=0.97; and MI vs. KPI, r=0.85. In nasal smears, the anucleate cell type was 
      found most frequently (about 75%) while intermediate cells with round or oval 
      nuclei comprised approximately 13% of the total smear composition. When donor 
      means for cell area and dry mass of buccal cells were combined for study, they 
      were highly correlated (r=0.88). Similarly, when the maturation indices for 
      buccal and nasal cells were combined for study, they were highly correlated: CDI 
      vs. MI, r=0.99; CDI vs. KPI, r=0.99; and MI vs. KPI, r=0.99. Of the three 
      indices, we believe that the CDI is the index best suited for use in studies of 
      cell development because it is based on an inferred sequence of cellular and 
      nuclear changes occurring during the maturation of epithelial cells. It provides 
      a more detailed description of the cells observed in the smears. It also 
      correlates highly in KPI and MI used in clinical and research efforts.
FAU - Pappelis, C K
AU  - Pappelis CK
FAU - Slobin, J
AU  - Slobin J
FAU - Corvallis, J
AU  - Corvallis J
FAU - Detwiler, H D
AU  - Detwiler HD
FAU - Pappelis, A J
AU  - Pappelis AJ
FAU - Pappelis, G A
AU  - Pappelis GA
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Switzerland
TA  - Acta Cytol
JT  - Acta cytologica
JID - 0370307
SB  - IM
MH  - Cell Nucleus/ultrastructure
MH  - Cheek
MH  - Cytological Techniques
MH  - Epithelial Cells
MH  - Humans
MH  - Microscopy, Interference
MH  - Mouth Mucosa/*cytology
MH  - Nasal Mucosa/*cytology
EDAT- 1976/07/01 00:00
MHDA- 1976/07/01 00:01
CRDT- 1976/07/01 00:00
PHST- 1976/07/01 00:00 [pubmed]
PHST- 1976/07/01 00:01 [medline]
PHST- 1976/07/01 00:00 [entrez]
PST - ppublish
SO  - Acta Cytol. 1976 Jul-Aug;20(4):372-4.