PMID- 7859561
OWN - NLM
STAT- MEDLINE
DCOM- 19950323
LR  - 20190830
IS  - 0009-5915 (Print)
IS  - 0009-5915 (Linking)
VI  - 103
IP  - 6
DP  - 1994 Oct
TI  - Visualization of mitotic chromosomes in filamentous fungi by fluorescence 
      staining and fluorescence in situ hybridization.
PG  - 408-13
AB  - Mitotic chromosomes of the plant pathogenic filamentous fungi Botrytis cinerea 
      and Alternaria alternata were observed. Chromosomes prepared by the germ tube 
      burst method were stained with the fluorescent dye 4',6-diamidino-2-phenylindole 
      (DAPI) to yield figures with good resolution. Using this method, component 
      chromosomes were clearly distinguished and the chromosome number could be 
      determined. Fluorescence in situ hybridization (FISH) was also successfully 
      applied to the specimens, revealing one ribosomal RNA gene cluster, or nucleolus 
      organizer region (NOR) in the genome of each fungus. A long attenuated chromatid 
      thread expanding from a condensed metaphase chromosome, which had been called a 
      thread-like structure in B. cinerea, was proved to be an NOR. This is the first 
      report of the successful application of FISH to the chromosomes of filamentous 
      fungi.
FAU - Taga, M
AU  - Taga M
AD  - College of General Education, Okayama University, Japan.
FAU - Murata, M
AU  - Murata M
LA  - eng
PT  - Journal Article
PL  - Austria
TA  - Chromosoma
JT  - Chromosoma
JID - 2985138R
RN  - 0 (DNA, Fungal)
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Indoles)
RN  - 47165-04-8 (DAPI)
SB  - IM
MH  - Alternaria/*ultrastructure
MH  - *Chromosomes, Fungal
MH  - DNA, Fungal/analysis
MH  - DNA, Ribosomal/analysis
MH  - Fluorescent Dyes
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Indoles
MH  - Karyotyping/methods
MH  - Mitosis
MH  - Mitosporic Fungi/*ultrastructure
MH  - Nucleolus Organizer Region/ultrastructure
EDAT- 1994/10/01 00:00
MHDA- 1994/10/01 00:01
CRDT- 1994/10/01 00:00
PHST- 1994/10/01 00:00 [pubmed]
PHST- 1994/10/01 00:01 [medline]
PHST- 1994/10/01 00:00 [entrez]
AID - 10.1007/BF00362285 [doi]
PST - ppublish
SO  - Chromosoma. 1994 Oct;103(6):408-13. doi: 10.1007/BF00362285.