PMID- 7861547
OWN - NLM
STAT- MEDLINE
DCOM- 19950320
LR  - 20220409
IS  - 0022-5347 (Print)
IS  - 0022-5347 (Linking)
VI  - 153
IP  - 3 Pt 1
DP  - 1995 Mar
TI  - PGE1 suppresses the induction of collagen synthesis by transforming growth 
      factor-beta 1 in human corpus cavernosum smooth muscle.
PG  - 826-34
AB  - Collagen synthesis has been examined in primary cultures of human corpus 
      cavernosum smooth muscle cells (HCCSMC), the major mesenchymal cell type of the 
      corpus cavernosum. These cultures were grown from human surgical specimens and 
      characterized by morphological and biochemical characteristics. These cells 
      express mRNA for transforming growth factor-beta 1 (TGF-beta 1), a major 
      regulator of extracellular matrix synthesis, as well as all three subtypes of 
      TGF-beta receptors. Human corpus cavernosum smooth muscle cells primarily 
      synthesize types I and III fibrillar collagen. Treatment of HCCSMC with exogenous 
      TGF-beta 1 (80 pM.) induced a 2.5- to 4.5-fold increase in the synthesis of types 
      I and III collagen and resulted in detectable levels of type V/XI collagen. 
      Treatment of HCCSMC with the eicosanoid PGE1 in combination with TGF-beta 1 
      suppressed the induction of collagen synthesis by TGF-beta 1 in a dose-dependent 
      manner with concomitant decreases in types I, III and V/XI collagen. The 
      expression of TGF-beta 1 mRNA as well as types I and II TGF-beta receptors was 
      induced by exogenous TGF-beta 1. Transforming growth factor-beta 1 mRNA induction 
      was suppressed by PGE1. These data suggest that prostaglandins and TGF-beta 1 may 
      play a key role in modulation of collagen synthesis in the corpus cavernosum, and 
      in the regulation of fibrosis of the corpus cavernosum.
FAU - Moreland, R B
AU  - Moreland RB
AD  - Department of Urology, Boston University School of Medicine, Massachusetts.
FAU - Traish, A
AU  - Traish A
FAU - McMillin, M A
AU  - McMillin MA
FAU - Smith, B
AU  - Smith B
FAU - Goldstein, I
AU  - Goldstein I
FAU - Saenz de Tejada, I
AU  - Saenz de Tejada I
LA  - eng
GR  - DK39080/DK/NIDDK NIH HHS/United States
GR  - DK4002505/DK/NIDDK NIH HHS/United States
GR  - DK40487/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Urol
JT  - The Journal of urology
JID - 0376374
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Transforming Growth Factor beta)
RN  - 0 (Transforming Growth Factor beta)
RN  - 9007-34-5 (Collagen)
RN  - F5TD010360 (Alprostadil)
SB  - IM
MH  - Alprostadil/*physiology
MH  - Cell Division
MH  - Cells, Cultured
MH  - Collagen/*biosynthesis
MH  - Humans
MH  - Male
MH  - Muscle, Smooth/cytology/*metabolism
MH  - Penis/cytology/*metabolism
MH  - Protein Biosynthesis
MH  - RNA, Messenger/biosynthesis
MH  - Receptors, Transforming Growth Factor beta/biosynthesis
MH  - Transforming Growth Factor beta/antagonists & inhibitors/genetics/*physiology
EDAT- 1995/03/01 00:00
MHDA- 1995/03/01 00:01
CRDT- 1995/03/01 00:00
PHST- 1995/03/01 00:00 [pubmed]
PHST- 1995/03/01 00:01 [medline]
PHST- 1995/03/01 00:00 [entrez]
AID - S0022-5347(01)67730-9 [pii]
PST - ppublish
SO  - J Urol. 1995 Mar;153(3 Pt 1):826-34.