PMID- 7861762
OWN - NLM
STAT- MEDLINE
DCOM- 19950323
LR  - 20171116
IS  - 0022-4804 (Print)
IS  - 0022-4804 (Linking)
VI  - 58
IP  - 2
DP  - 1995 Feb
TI  - Tumor necrosis factor-alpha and interleukin-6 selectively regulate neutrophil 
      function in vitro.
PG  - 124-30
AB  - The neutrophil is an important effector cell of the host response to sepsis. 
      Tumor necrosis factor-alpha (TNF-alpha), a cytokine mediator of the septic 
      response, is rapidly released following endotoxemia or gram-negative bacteremia. 
      Interleukin-6 (IL-6) is another cytokine mediator of the host response to sepsis 
      whose role is less well understood than that of TNF-alpha. It is known to be 
      elevated in gram-negative sepsis, where peak levels have been correlated with 
      mortality. This study examined the effect of IL-6 alone and in combination with 
      TNF-alpha on three neutrophil functions--CD18 adhesion receptor expression, 
      phagocytosis, and superoxide anion generation. Neutrophils from human volunteers 
      were incubated with amounts of IL-6 ranging from 10 to 1000 ng/ml. At a 
      concentration of 1000 ng/ml, IL-6 increased neutrophil phagocytosis of opsonized 
      bacteria (826 +/- 255 x 10(3) MESF vs 552 +/- 103 MESF, P < 0.05) and also 
      increased neutrophil superoxide anion generation (18.41 +/- 1.86 vs 12.6 nmol 
      O2-/10(6) PMN/10 min, P < 0.05). Lesser amounts of IL-6 had no effect on 
      phagocytosis or superoxide generation. IL-6 did not increase neutrophil CD18 
      adhesion receptor expression. Combining IL-6 with TNF-alpha at doses of 100 ng/ml 
      and 100 U/ml, respectively, neutrophil phagocytosis (221 +/- 455 MESF vs 552 +/- 
      103 MESF) and superoxide generation (23.18 +/- 1.86 vs 12.6 nmol O2-/10(6) PMN/10 
      min) were significantly (P < 0.05) increased above control by an amount similar 
      to that seen with 1000 U/ml TNF-alpha alone.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Mullen, P G
AU  - Mullen PG
AD  - Department of Surgery, Medical College of Virginia--Virginia Commonwealth 
      University, Richmond 23298.
FAU - Windsor, A C
AU  - Windsor AC
FAU - Walsh, C J
AU  - Walsh CJ
FAU - Fowler, A A 3rd
AU  - Fowler AA 3rd
FAU - Sugerman, H J
AU  - Sugerman HJ
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Surg Res
JT  - The Journal of surgical research
JID - 0376340
RN  - 0 (CD18 Antigens)
RN  - 0 (Interleukin-6)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 11062-77-4 (Superoxides)
SB  - IM
MH  - Adult
MH  - CD18 Antigens/analysis
MH  - Humans
MH  - Interleukin-6/*pharmacology
MH  - Male
MH  - Neutrophils/*drug effects/physiology
MH  - Phagocytosis/drug effects
MH  - Superoxides/metabolism
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 1995/02/01 00:00
MHDA- 2001/03/28 10:01
CRDT- 1995/02/01 00:00
PHST- 1995/02/01 00:00 [pubmed]
PHST- 2001/03/28 10:01 [medline]
PHST- 1995/02/01 00:00 [entrez]
AID - S0022-4804(85)71020-7 [pii]
AID - 10.1006/jsre.1995.1020 [doi]
PST - ppublish
SO  - J Surg Res. 1995 Feb;58(2):124-30. doi: 10.1006/jsre.1995.1020.