PMID- 7865231
OWN - NLM
STAT- MEDLINE
DCOM- 19950328
LR  - 20190904
IS  - 0284-186X (Print)
IS  - 0284-186X (Linking)
VI  - 34
IP  - 1
DP  - 1995
TI  - Detection of structural aberrations of chromosome 17 in malignant gliomas by 
      fluorescence in situ hybridization (FISH).
PG  - 27-30
AB  - Isolated nuclei prepared from fresh-frozen tissue specimens were used to 
      investigate structural aberrations of chromosome 17 in 18 malignant gliomas (7 
      anaplastic astrocytomas, 11 glioblastomas). Nuclear DNA was hybridized with four 
      DNA probes specific for centromeric (D17ZI), telomeric (Tel 17p, Tel 17q) and 17p 
      13.1 sequences (p53) of chromosome 17, using fluorescence in situ hybridization 
      (FISH). The rates of nuclei with one signal (OS) for Tel 17p and p53 were 
      significantly higher than those for D17ZI and Tel 17q. This indicated that the 
      majority of chromosome 17 aberrations was a deletion of the short arm including 
      the p53 gene. When compared with the histological grading, the rates of OS for 
      Tel 17p and p53 in anaplastic astrocytomas were higher than those of 
      glioblastomas, suggesting that the deletion may be associated with the early 
      events in tumorigenesis and that some glioblastomas without chromosome 17 
      aberrations may be independent from tumour progression via low-grade gliomas.
FAU - Kwak, T
AU  - Kwak T
AD  - Department of Neurosurgery, Yamaguchi University School of Medicine, Japan.
FAU - Nishizaki, T
AU  - Nishizaki T
FAU - Nakayama, H
AU  - Nakayama H
FAU - Kakino, S
AU  - Kakino S
FAU - Ito, H
AU  - Ito H
FAU - Kimura, Y
AU  - Kimura Y
LA  - eng
PT  - Journal Article
PL  - Sweden
TA  - Acta Oncol
JT  - Acta oncologica (Stockholm, Sweden)
JID - 8709065
RN  - 0 (DNA Probes)
RN  - 0 (DNA, Neoplasm)
SB  - IM
GS  - p53
MH  - Astrocytoma/genetics
MH  - Centromere
MH  - Chromosome Aberrations/genetics
MH  - *Chromosome Deletion
MH  - *Chromosomes, Human, Pair 17
MH  - DNA Probes
MH  - DNA, Neoplasm
MH  - Genes, p53
MH  - Glioblastoma/genetics
MH  - Glioma/*genetics/pathology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - Telomere
EDAT- 1995/01/01 00:00
MHDA- 1995/01/01 00:01
CRDT- 1995/01/01 00:00
PHST- 1995/01/01 00:00 [pubmed]
PHST- 1995/01/01 00:01 [medline]
PHST- 1995/01/01 00:00 [entrez]
AID - 10.3109/02841869509093634 [doi]
PST - ppublish
SO  - Acta Oncol. 1995;34(1):27-30. doi: 10.3109/02841869509093634.