PMID- 7866434
OWN - NLM
STAT- MEDLINE
DCOM- 19950330
LR  - 20161123
IS  - 0968-8773 (Print)
IS  - 0968-8773 (Linking)
VI  - 40
IP  - 4
DP  - 1994
TI  - Hepatocyte growth factor (HGF), keratinocyte growth factor (KGF), and their 
      receptors in human breast cells and tissues: alternative receptors.
PG  - 337-50
AB  - We sought to determine whether the hepatocyte growth factor/scatter factor 
      (HGF/SF)- and keratinocyte growth factor-receptor systems were expressed in 
      normal breast cells, breast carcinoma cell lines, normal breast tissues, and 
      breast cancer tissues. Reverse transcriptase-polymerase chain reaction and hot 
      blotting were used to detect HGF, HGF/SF (met) receptor, KGF, and KGF receptor 
      mRNAs in human mammary epithelial (HME) and stromal (HMS) cells. We also examined 
      breast carcinoma (MDA-MB-157, SCC 38, and SCC 70) and spontaneously immortalized 
      breast epithelial (HMT 3522) cell lines, as well as normal breast and breast 
      carcinoma tissues. PCR products were also confirmed by nucleic acid sequencing. 
      The effects of HGF and KGF, compared to EGF and heparin-binding EGF, on the 
      proliferation of normal human mammary epithelial cells in serum-free defined 
      medium was determined by cell counting. HGF and KGF mRNAs were detected in HMS 
      cells, but not HME cells. KGF receptor mRNA was detected in HME cells, but not 
      HMS cells. HGF/SF receptor mRNA was detected in both HME and HMS cells. mRNAs 
      were also detected in normal breast and breast carcinoma tissues, as well as 
      breast carcinoma and transformed breast epithelial cell lines. Alternative cDNA 
      sequences that are predicted to code for a soluble KGF receptor and a membrane 
      bound, truncated HGF/SF receptor were detected in breast epithelial cells and 
      breast tissues. HGF and KGF maintained viability and stimulated proliferation of 
      HME cells.
FAU - Wilson, S E
AU  - Wilson SE
AD  - Department of Ophthalmology, University of Texas Southwestern Medical Center at 
      Dallas 75235.
FAU - Weng, J
AU  - Weng J
FAU - Chwang, E L
AU  - Chwang EL
FAU - Gollahon, L
AU  - Gollahon L
FAU - Leitch, A M
AU  - Leitch AM
FAU - Shay, J W
AU  - Shay JW
LA  - eng
SI  - GENBANK/U11813
SI  - GENBANK/U11814
GR  - CA64871/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cell Mol Biol Res
JT  - Cellular & molecular biology research
JID - 9316986
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (FGF7 protein, human)
RN  - 0 (Fibroblast Growth Factor 10)
RN  - 0 (Growth Substances)
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Fibroblast Growth Factor)
RN  - 0 (Receptors, Growth Factor)
RN  - 126469-10-1 (Fibroblast Growth Factor 7)
RN  - 62031-54-3 (Fibroblast Growth Factors)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9007-49-2 (DNA)
RN  - EC 2.7.10.1 (Proto-Oncogene Proteins c-met)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.10.1 (Receptor, Fibroblast Growth Factor, Type 2)
RN  - EC 2.7.10.1 (keratinocyte growth factor receptor)
SB  - IM
EIN - Cell Mol Biol Res 1994;40(7-8):707
MH  - Adult
MH  - Aged
MH  - Alternative Splicing
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Breast/cytology/*metabolism
MH  - Breast Neoplasms/metabolism
MH  - Cattle
MH  - Cell Line
MH  - Cell Survival
MH  - Culture Media, Serum-Free
MH  - DNA
MH  - Epidermal Growth Factor/pharmacology
MH  - Epithelial Cells
MH  - Epithelium/metabolism
MH  - Female
MH  - Fibroblast Growth Factor 10
MH  - Fibroblast Growth Factor 7
MH  - *Fibroblast Growth Factors
MH  - *Gene Expression Regulation, Neoplastic
MH  - Growth Substances/*biosynthesis/genetics/pharmacology
MH  - Hepatocyte Growth Factor/*biosynthesis/genetics/pharmacology
MH  - Humans
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Proto-Oncogene Proteins c-met
MH  - RNA, Messenger/biosynthesis
MH  - Receptor Protein-Tyrosine Kinases/*biosynthesis/genetics
MH  - Receptor, Fibroblast Growth Factor, Type 2
MH  - *Receptors, Fibroblast Growth Factor
MH  - Receptors, Growth Factor/*biosynthesis/genetics
MH  - Stromal Cells
MH  - Tumor Cells, Cultured
EDAT- 1994/01/01 00:00
MHDA- 1994/01/01 00:01
CRDT- 1994/01/01 00:00
PHST- 1994/01/01 00:00 [pubmed]
PHST- 1994/01/01 00:01 [medline]
PHST- 1994/01/01 00:00 [entrez]
PST - ppublish
SO  - Cell Mol Biol Res. 1994;40(4):337-50.