PMID- 7867908
OWN - NLM
STAT- MEDLINE
DCOM- 19950324
LR  - 20190909
IS  - 0272-0590 (Print)
IS  - 0272-0590 (Linking)
VI  - 23
IP  - 4
DP  - 1994 Nov
TI  - Evaluation of an azo and two anthraquinone dyes for allergic potential.
PG  - 569-77
AB  - Two dye mixtures and the individual component dyes were evaluated for the 
      potential to induce contact or pulmonary hypersensitivity. These dye mixtures 
      were suspect because of anecdotal reports of both pulmonary and contact 
      hypersensitivity in assembly workers, and because the component dyes were 
      structurally related to dyes known to be contact sensitizers. One mixture 
      consisted of disperse blue 3 (DB3) and disperse red 11 (DR11), which are 
      anthraquinones, and the other mixture contained DR11 and solvent red 1 (SR1), an 
      azo dye. Contact hypersensitivity was examined using the local lymph node assay 
      (LLNA) and a modified mouse ear swelling test (MEST). Both the MEST and the LLNA 
      indicated that SR1 has weak contact-sensitizing potential. None of the other 
      individual dye compounds or the two mixtures were identified as contact 
      sensitizers by either method. To evaluate the mixtures as potential pulmonary 
      allergens, guinea pigs were repeatedly exposed by inhalation (300 mg/m3, 6 
      hr/day) 5 days/week, for 1 week. Weekly exposures were repeated three times with 
      2 weeks of nonexposure time in between. Guinea pigs were then challenged through 
      the jugular vein using a dye-dimethylsulfoxide mixture. During the challenge, 
      breathing mechanics (dynamic compliance and resistance) were measured in 
      mechanically ventilated animals. Changes in these measurements, indicative of 
      bronchoconstriction, were not observed in animals exposed to either dye mixture, 
      nor were antibodies detected in the sera of exposed animals using individual 
      dye-specific enzyme-linked immunosorbent assays. In conclusion, two methods 
      indicate that SR1 may have contact-sensitizing potential.(ABSTRACT TRUNCATED AT 
      250 WORDS)
FAU - Sailstad, D M
AU  - Sailstad DM
AD  - ManTech Environmental Technology, Inc., Research Triangle Park, North Carolina 
      27709.
FAU - Tepper, J S
AU  - Tepper JS
FAU - Doerfler, D L
AU  - Doerfler DL
FAU - Qasim, M
AU  - Qasim M
FAU - Selgrade, M K
AU  - Selgrade MK
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Fundam Appl Toxicol
JT  - Fundamental and applied toxicology : official journal of the Society of 
      Toxicology
JID - 8200838
RN  - 0 (Anthraquinones)
RN  - 0 (Azo Compounds)
RN  - 0 (Coloring Agents)
SB  - IM
MH  - Animals
MH  - Anthraquinones/toxicity
MH  - Azo Compounds/toxicity
MH  - Coloring Agents/administration & dosage/*toxicity
MH  - Dermatitis, Allergic Contact/*etiology
MH  - Ear, External/pathology
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Female
MH  - Guinea Pigs
MH  - Lymph Nodes/drug effects
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Respiratory Hypersensitivity/*chemically induced
EDAT- 1994/11/01 00:00
MHDA- 1994/11/01 00:01
CRDT- 1994/11/01 00:00
PHST- 1994/11/01 00:00 [pubmed]
PHST- 1994/11/01 00:01 [medline]
PHST- 1994/11/01 00:00 [entrez]
AID - S0272059084711420 [pii]
AID - 10.1006/faat.1994.1142 [doi]
PST - ppublish
SO  - Fundam Appl Toxicol. 1994 Nov;23(4):569-77. doi: 10.1006/faat.1994.1142.