PMID- 7870576
OWN - NLM
STAT- MEDLINE
DCOM- 19950328
LR  - 20190501
IS  - 0305-1048 (Print)
IS  - 1362-4962 (Electronic)
IS  - 0305-1048 (Linking)
VI  - 23
IP  - 1
DP  - 1995 Jan 11
TI  - Construction of a genomic DNA 'feature map' by sequencing from nested deletions: 
      application to the HLA class I region.
PG  - 117-22
AB  - We are applying a transposon-based approach for detecting and mapping features of 
      special interest to construct 'feature maps' of currently uncharacterized 
      portions of the human leukocyte antigen (HLA) complex on chromosome 6. Such 
      feature maps should facilitate identifying regions for high resolution analysis. 
      Here we describe the feature mapping of a 35 kb DNA fragment located between the 
      HLA-C and HLA-E loci. This fragment was cloned into a transposon gamma 
      delta-based cosmid vector designed for generating nested deletions in vivo. 
      Seventy informative nested deletions extending into the cloned fragment were 
      isolated, and DNA adjacent to the deletion endpoints was sequenced by fluorescent 
      automated technology. These islands of DNA sequences constituted the foundation 
      of the feature map, and (i) identified putative exons, (ii) determined the 
      positions of Alu elements, (iii) determined the span of the keratinocyte-specific 
      S gene, and (iv) localized evolutionarily conserved sequences. The construction 
      of feature maps using this in vivo nested deletion-sequencing approach provides a 
      rapid and efficient means to identify DNA regions that merit more detailed 
      analysis.
FAU - Krishnan, B R
AU  - Krishnan BR
AD  - Department of Internal Medicine, Howard Hughes Medical Institute and Department 
      of Molecular Microbiology, Washington University School of Molecular 
      Microbiology, Washington University School of Medicine, St Louis, MO 63110.
FAU - Jamry, I
AU  - Jamry I
FAU - Berg, D E
AU  - Berg DE
FAU - Berg, C M
AU  - Berg CM
FAU - Chaplin, D D
AU  - Chaplin DD
LA  - eng
SI  - GENBANK/U18138
SI  - GENBANK/U18139
SI  - GENBANK/U18140
SI  - GENBANK/U18141
SI  - GENBANK/U18142
SI  - GENBANK/U18143
SI  - GENBANK/U18144
SI  - GENBANK/U18145
SI  - GENBANK/U18146
SI  - GENBANK/U18147
SI  - GENBANK/U18148
SI  - GENBANK/U18149
SI  - GENBANK/U18150
SI  - GENBANK/U18151
SI  - GENBANK/U18152
SI  - GENBANK/U18153
SI  - GENBANK/U18154
SI  - GENBANK/U18155
SI  - GENBANK/U18156
SI  - GENBANK/U18157
SI  - GENBANK/U18158
SI  - GENBANK/U18159
SI  - GENBANK/U18160
SI  - GENBANK/U18161
SI  - GENBANK/U18162
SI  - GENBANK/U18163
SI  - GENBANK/U18166
SI  - GENBANK/U18167
SI  - GENBANK/U18168
SI  - GENBANK/U18169
GR  - AI15322/AI/NIAID NIH HHS/United States
GR  - AI34580/AI/NIAID NIH HHS/United States
GR  - HG00563/HG/NHGRI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - England
TA  - Nucleic Acids Res
JT  - Nucleic acids research
JID - 0411011
RN  - 0 (DNA Primers)
RN  - 0 (DNA Transposable Elements)
RN  - 0 (HLA Antigens)
RN  - 9007-49-2 (DNA)
SB  - IM
GS  - HLA-C
MH  - Base Sequence
MH  - Biological Evolution
MH  - *Chromosome Mapping
MH  - Chromosomes, Human, Pair 6
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - Cosmids
MH  - DNA/*genetics
MH  - DNA Primers/genetics
MH  - DNA Transposable Elements
MH  - Exons
MH  - Genetic Vectors
MH  - Genome, Human
MH  - HLA Antigens/*genetics
MH  - Humans
MH  - Molecular Sequence Data
MH  - Multigene Family
MH  - Repetitive Sequences, Nucleic Acid
MH  - Sequence Deletion
PMC - PMC306638
EDAT- 1995/01/11 00:00
MHDA- 1995/01/11 00:01
CRDT- 1995/01/11 00:00
PHST- 1995/01/11 00:00 [pubmed]
PHST- 1995/01/11 00:01 [medline]
PHST- 1995/01/11 00:00 [entrez]
AID - 4s0637 [pii]
AID - 10.1093/nar/23.1.117 [doi]
PST - ppublish
SO  - Nucleic Acids Res. 1995 Jan 11;23(1):117-22. doi: 10.1093/nar/23.1.117.