PMID- 7871055
OWN - NLM
STAT- MEDLINE
DCOM- 19950329
LR  - 20190726
IS  - 0033-3158 (Print)
IS  - 0033-3158 (Linking)
VI  - 112
IP  - 4
DP  - 1993
TI  - Cocaine binding sites in fetal rat brain: implications for prenatal cocaine 
      action.
PG  - 445-51
AB  - Binding of [3H]cocaine to membrane preparations from whole fetal rat brain was 
      studied. High-affinity binding (10 nM cocaine) was detected as early as 
      gestational day (GD) 15 and steadily increased across subsequent development. 
      Saturation studies comparing [3H]cocaine binding at GD20 and adulthood yielded 
      similar KD values, and LIGAND analyses favored a two-site model if an extended 
      range of [3H]cocaine concentrations was used. Various monoamine uptake inhibitors 
      displaced labeled cocaine with potencies consistent with the idea that 
      [3H]cocaine labels the dopamine (DA), serotonin (5-HT), and possibly also the 
      norepinephrine (NE) transporters in whole fetal brain preparations. Synaptosomal 
      DA uptake was well developed by GD20, as was the potency of cocaine to inhibit 
      such uptake. The results indicate that functional, monoamine transporter related 
      cocaine binding sites are present in the fetal rat brain. Such sites are likely 
      to play an important role in mediating the direct interactions of 
      prenatally-administered cocaine with developing monoaminergic systems in both 
      animals and humans.
FAU - Meyer, J S
AU  - Meyer JS
AD  - Department of Psychology, University of Massachusetts, Amherst 01003.
FAU - Shearman, L P
AU  - Shearman LP
FAU - Collins, L M
AU  - Collins LM
FAU - Maguire, R L
AU  - Maguire RL
LA  - eng
GR  - DA06495/DA/NIDA NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - Germany
TA  - Psychopharmacology (Berl)
JT  - Psychopharmacology
JID - 7608025
RN  - 0 (Neurotransmitter Uptake Inhibitors)
RN  - 0 (Receptors, Drug)
RN  - 333DO1RDJY (Serotonin)
RN  - I5Y540LHVR (Cocaine)
RN  - VTD58H1Z2X (Dopamine)
RN  - X4W3ENH1CV (Norepinephrine)
SB  - IM
MH  - Animals
MH  - Brain/drug effects/embryology/*metabolism
MH  - Cocaine/*metabolism/pharmacokinetics
MH  - Dopamine/metabolism
MH  - Female
MH  - In Vitro Techniques
MH  - Male
MH  - Membranes/drug effects/metabolism
MH  - Neurotransmitter Uptake Inhibitors/pharmacology
MH  - Norepinephrine/metabolism
MH  - Pregnancy
MH  - *Prenatal Exposure Delayed Effects
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Drug/*metabolism
MH  - Serotonin/metabolism
MH  - Synaptosomes/drug effects/metabolism
EDAT- 1993/01/01 00:00
MHDA- 1993/01/01 00:01
CRDT- 1993/01/01 00:00
PHST- 1993/01/01 00:00 [pubmed]
PHST- 1993/01/01 00:01 [medline]
PHST- 1993/01/01 00:00 [entrez]
AID - 10.1007/BF02244892 [doi]
PST - ppublish
SO  - Psychopharmacology (Berl). 1993;112(4):445-51. doi: 10.1007/BF02244892.