PMID- 7875039
OWN - NLM
STAT- MEDLINE
DCOM- 19950404
LR  - 20071114
IS  - 0196-4763 (Print)
IS  - 0196-4763 (Linking)
VI  - 17
IP  - 4
DP  - 1994 Dec 1
TI  - Bladder irrigation specimens assayed by fluorescence in situ hybridization to 
      interphase nuclei.
PG  - 319-26
AB  - Bladder irrigation specimens provide a sampling of the entire bladder urothelium 
      and are the most practical sample for longitudinally monitoring patients. This 
      study presents cross-sectional fluorescence in situ hybridization (FISH) analyses 
      with correlated DNA cytometry data on 76 patients monitored for recurrent bladder 
      tumors. FISH probes complementary to centromeric satellite sequences for 
      chromosomes 1, 7, 9, 11, 15, and 17 were used. Aberrations in copy number were 
      observed for chromosomes 1, 7, 11, and 17 principally in patients with aneuploid 
      tumors. Monosomy of chromosome 9 was observed in 39% of the diploid and 31% of 
      the specimens with high hyperdiploid fraction. Significantly, 24% of patients 
      with a history of bladder cancer but with no clinical evidence of disease 
      exhibited monosomy of chromosome 9. This suggests a persistent and significantly 
      large population of abnormal cells in the absence of clinical evidence of 
      disease. Loss of chromosome 9 relative to DNA ploidy was observed in 24% of 
      patients with no evidence of disease, in 59% of patients with tumor, and in 79% 
      of patients with histologically confirmed transitional cell carcinoma, grades 
      1-3. Loss of chromosome 15 was also observed in a large percentage of patients. 
      Loss of chromosome 15 was observed in 41% of specimens from patients in whom no 
      tumor was seen, in 38% of specimens from patients with tumor, and in 67% of 
      specimens from patients with histologically confirmed transitional cell 
      carcinoma. Results of this study document the use of bladder irrigation specimens 
      as a specimen source for FISH analyses.(ABSTRACT TRUNCATED AT 250 WORDS)
FAU - Wheeless, L L
AU  - Wheeless LL
AD  - Department of Pathology and Laboratory Medicine, University of Rochester Medical 
      Center, New York 14642.
FAU - Reeder, J E
AU  - Reeder JE
FAU - Han, R
AU  - Han R
FAU - O'Connell, M J
AU  - O'Connell MJ
FAU - Frank, I N
AU  - Frank IN
FAU - Cockett, A T
AU  - Cockett AT
FAU - Hopman, A H
AU  - Hopman AH
LA  - eng
GR  - R01 CA33148/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cytometry
JT  - Cytometry
JID - 8102328
RN  - 0 (DNA, Neoplasm)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Base Sequence
MH  - Child
MH  - *Chromosome Aberrations/genetics
MH  - Chromosomes, Human, Pair 1/genetics
MH  - Chromosomes, Human, Pair 11/genetics
MH  - *Chromosomes, Human, Pair 15/genetics
MH  - Chromosomes, Human, Pair 17/genetics
MH  - Chromosomes, Human, Pair 7/genetics
MH  - *Chromosomes, Human, Pair 9/genetics
MH  - DNA, Neoplasm/*analysis
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/methods
MH  - Interphase/genetics
MH  - Male
MH  - Middle Aged
MH  - Molecular Sequence Data
MH  - Urinary Bladder/*pathology
MH  - Urinary Bladder Neoplasms/genetics/*pathology
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - 10.1002/cyto.990170407 [doi]
PST - ppublish
SO  - Cytometry. 1994 Dec 1;17(4):319-26. doi: 10.1002/cyto.990170407.