PMID- 7876234
OWN - NLM
STAT- MEDLINE
DCOM- 19950405
LR  - 20231213
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 270
IP  - 9
DP  - 1995 Mar 3
TI  - Molecular cloning of a human genomic region containing the H blood group 
      alpha(1,2)fucosyltransferase gene and two H locus-related DNA restriction 
      fragments. Isolation of a candidate for the human Secretor blood group locus.
PG  - 4632-9
AB  - We have used the human H blood group alpha(1,2)fucosyltransferase (FUT1) cDNA to 
      screen chromosome 19 cosmid libraries in a search for the human Secretor (Se) 
      blood group gene (FUT2). One cosmid has been isolated that contains two distinct 
      segments that cross-hybridize with FUT1. We have assembled a 100-kilobase (kb) 
      cosmid contig, localized to 19q13.3, encompassing FUT1 and the two FUT1-related 
      sequences, termed Sec1 and Sec2, for Secretor candidate 1 and 2. Sec1 and Sec2 
      are separated by 12 kb and are 65.5 kb and 35 kb apart, respectively, from the 
      FUT1 gene. We used a cosmid-dependent direct cDNA selection method to clone a 
      cDNA corresponding to a transcript that emanates from Sec2. This cDNA detects a 
      3.35-kb transcript in human tissues known to express the Se locus. Together with 
      sequence and expression data reported in the accompanying article (Kelly, R. J., 
      Rouquier, S., Giorgi, D., Lennon, G. G., and Lowe, J. B. (1995) J. Biol. Chem. 
      270, 4640-4649), these data demonstrate that Sec2 corresponds to the human Se 
      blood group locus (FUT2). Our results furthermore define the physical 
      relationship between the H and Se loci and confirm a hypothesis that these two 
      loci represent distinct but closely linked alpha(1,2)fucosyltransferase genes.
FAU - Rouquier, S
AU  - Rouquier S
AD  - Human Genome Center, Lawrence Livermore National Laboratory, Livermore, 
      California 94550.
FAU - Lowe, J B
AU  - Lowe JB
FAU - Kelly, R J
AU  - Kelly RJ
FAU - Fertitta, A L
AU  - Fertitta AL
FAU - Lennon, G G
AU  - Lennon GG
FAU - Giorgi, D
AU  - Giorgi D
LA  - eng
GR  - 1R01HL48859/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (Blood Group Antigens)
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - EC 2.4.1.- (Fucosyltransferases)
RN  - EC 3.1.21.- (Deoxyribonuclease EcoRI)
SB  - IM
GS  - FUT1
MH  - Animals
MH  - Blood Group Antigens/*genetics
MH  - Chromosomes, Artificial, Yeast
MH  - Chromosomes, Human, Pair 19
MH  - Cloning, Molecular
MH  - Cosmids
MH  - Cricetinae
MH  - Cricetulus
MH  - DNA, Complementary/genetics
MH  - Deoxyribonuclease EcoRI
MH  - Fucosyltransferases/*genetics
MH  - Genome, Human
MH  - Humans
MH  - Hybrid Cells
MH  - In Situ Hybridization, Fluorescence
MH  - Intestinal Mucosa/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Restriction Mapping
MH  - Galactoside 2-alpha-L-fucosyltransferase
EDAT- 1995/03/03 00:00
MHDA- 1995/03/03 00:01
CRDT- 1995/03/03 00:00
PHST- 1995/03/03 00:00 [pubmed]
PHST- 1995/03/03 00:01 [medline]
PHST- 1995/03/03 00:00 [entrez]
AID - S0021-9258(18)82822-8 [pii]
AID - 10.1074/jbc.270.9.4632 [doi]
PST - ppublish
SO  - J Biol Chem. 1995 Mar 3;270(9):4632-9. doi: 10.1074/jbc.270.9.4632.