PMID- 7876284
OWN - NLM
STAT- MEDLINE
DCOM- 19950406
LR  - 20061115
IS  - 0021-9304 (Print)
IS  - 0021-9304 (Linking)
VI  - 28
IP  - 12
DP  - 1994 Dec
TI  - Osteoblast function on synthetic biodegradable polymers.
PG  - 1445-53
AB  - Rat osteoblasts were cultured on films of biodegradable poly(L-lactic acid) 
      (PLLA), 75:25 poly(DL-lactic-co-glycolic acid) (PLGA), 50:50 PLGA, and 
      poly(glycolic acid) (PGA) for up to 14 days. Osteoblasts attached equally well to 
      all the polymer substrates after 8 h in culture. By day 4 in culture, osteoblasts 
      had exceeded confluency numbers, and their proliferation leveled off by day 7. An 
      increase in alkaline phosphatase (ALP) activity from 1.92 (+/- 0.47) x 10(-7) for 
      day 7 to 5.75 (+/- 0.12) x 10(-7) mumol/cell per min for day 14 was reported for 
      osteoblasts cultured on 75:25 PLGA, which was comparable to that observed for 
      tissue culture polystyrene (TCPS) controls. The ALP activities expressed by 
      osteoblasts cultured on PLLA, 50:50 PLGA, and PGA films did not significantly 
      increase over time. Collagen synthesis for osteoblasts cultured on all polymer 
      substrates was similar to that of TCPS and did not vary with time. The morphology 
      of cultured osteoblasts was not affected by the continuous degradation of the 
      polymer substrates. These results demonstrate that poly(alpha-hydroxy esters) can 
      provide a suitable substrate for osteoblast culture and hold promise in bone 
      regeneration by osteoblast transplantation.
FAU - Ishaug, S L
AU  - Ishaug SL
AD  - Department of Chemical Engineering, Rice University, Houston, Texas 77251.
FAU - Yaszemski, M J
AU  - Yaszemski MJ
FAU - Bizios, R
AU  - Bizios R
FAU - Mikos, A G
AU  - Mikos AG
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - J Biomed Mater Res
JT  - Journal of biomedical materials research
JID - 0112726
RN  - 0 (Biocompatible Materials)
RN  - 0 (Polymers)
RN  - 9007-34-5 (Collagen)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Biocompatible Materials
MH  - Cell Adhesion
MH  - Cell Division
MH  - Cells, Cultured
MH  - Collagen/biosynthesis
MH  - Feasibility Studies
MH  - Microscopy, Electron, Scanning
MH  - Osteoblasts/cytology/metabolism/*physiology
MH  - *Polymers
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Time Factors
OTO - NASA
OT  - Non-programmatic
EDAT- 1994/12/01 00:00
MHDA- 1994/12/01 00:01
CRDT- 1994/12/01 00:00
PHST- 1994/12/01 00:00 [pubmed]
PHST- 1994/12/01 00:01 [medline]
PHST- 1994/12/01 00:00 [entrez]
AID - 10.1002/jbm.820281210 [doi]
PST - ppublish
SO  - J Biomed Mater Res. 1994 Dec;28(12):1445-53. doi: 10.1002/jbm.820281210.